Institute of Inorganic and Analytical Chemistry, Heinrich-Buff-Ring 17, Justus Liebig University, 35392 Giessen, Germany.
Institute of Inorganic and Analytical Chemistry, Heinrich-Buff-Ring 17, Justus Liebig University, 35392 Giessen, Germany.
Talanta. 2018 Feb 1;178:780-787. doi: 10.1016/j.talanta.2017.10.021. Epub 2017 Oct 12.
Organic UV filters in personal care products (PCPs) have been persistently reported as a potential threat to human health. In order to guarantee consumers safety, the dose of these compounds in PCPs needs to be monitored. Here, a methodology based on reactive low temperature plasma ionization (LTP) mass spectrometry (MS) has been developed for the determination of common organic UV filters in PCPs including benzophenone-3, ethylhexyl dimethyl p-aminobenzoic acid, ethylhexyl methoxycinnamate, 4-methylbenzylidene camphor, octocrylene, and ethylhexyl salicylate. The experiments were carried out in transmission geometry where the LTP ion source, samples loaded on a stainless steel mesh, and the MS inlet were aligned coaxially. Four chemicals, ammonia, ammonium formate, aniline, and methylamine were considered as reactive additives allowing reactions with the UV filters through different mechanisms. Methylamine-induced reactive LTP-MS showed the most prominent improvement on the detection of UV filter compounds. Compared to direct LTP-MS, the developed method improved the detection limits of UV filters more than 10 fold. Moreover, the method enabled fast semi-quantitative screening of UV filters in authentic PCPs. Concentrations of active ingredients in eight authentic PCPs as determined with reactive LTP-MS were found comparable to values offered by the cosmetic companies and corresponding HPLC data. The methodology provides high throughput analysis (70s per sample) and sensitive identification of organic UV filters. Lowest detectable concentrations ranged from 0.13µg/g for 4-methylbenzylidene camphor to 7.67µg/g for octocrylene in spiked cream. In addition, it shows the potential to be used as a screening tool for legal authentications of these chemicals in the future due to its semi-quantitative determination of UV filters in PCPs without tedious sample preparation and time-consuming chromatographic separation.
个人护理产品（PCP）中的有机紫外线滤光剂一直被认为是对人类健康的潜在威胁。为了保证消费者的安全，需要监测这些化合物在 PCP 中的剂量。在此，开发了一种基于反应低温等离子体电离（LTP）质谱（MS）的方法，用于测定 PCP 中常见的有机紫外线滤光剂，包括二苯酮-3、乙基己基二甲氨基苯甲酸酯、乙基己基甲氧基肉桂酸酯、4-甲基亚苄基樟脑、奥克立林和乙基己基水杨酸酯。实验在传输几何形状下进行，其中 LTP 离子源、加载在不锈钢网格上的样品和 MS 入口同轴对齐。考虑了四种化学物质，即氨、甲酸铵、苯胺和甲胺，它们作为反应性添加剂，通过不同的机制与紫外线滤光剂发生反应。甲胺诱导的反应性 LTP-MS 对检测紫外线滤光剂化合物的效果最为显著。与直接 LTP-MS 相比，所开发的方法将紫外线滤光剂的检测限提高了 10 倍以上。此外，该方法能够快速对真实 PCP 中的紫外线滤光剂进行半定量筛选。用反应性 LTP-MS 测定的八种真实 PCP 中的活性成分浓度与化妆品公司提供的值和相应的 HPLC 数据相当。该方法提供高通量分析（每个样品 70 秒）和对有机紫外线滤光剂的敏感识别。在加标霜中，最低检测浓度范围从 4-甲基亚苄基樟脑的 0.13µg/g 到奥克立林的 7.67µg/g。此外，由于其无需繁琐的样品制备和耗时的色谱分离即可对半定量测定 PCP 中的紫外线滤光剂，因此它有可能成为未来用于这些化学物质的法定认证的筛选工具。
