Key Laboratory for Advanced Materials, Institute of Fine Chemicals and School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
Key Laboratory for Advanced Materials, Institute of Fine Chemicals and School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
Talanta. 2018 Feb 1;178:847-853. doi: 10.1016/j.talanta.2017.10.026. Epub 2017 Oct 18.
Citrate is an important intermediate in the citric acid cycle, a vital metabolic pathway for animals, plants and bacteria. It is of great significance to detect its levels in human beings because several diseases may cause the abnormal of citrate. In this paper, a new turn-on fluorescent sensor (TPE-Py) using the classic tetraphenylethylene (TPE) as the aggregation-induced emission (AIE) fluorophore and bipyridinium-based amides as the recognition receptor has been synthesized for the detection of citrate. The probe exhibits good selectivity and sensitivity to citrate with a relatively low detection limit (1.0 × 10M). The enhancement of the fluorescence is relevant with the AIE property based on the complexation of TPE-Py with citrate caused by the hydrogen bonding and electrostatic interactions between the bipyridinium diamides and citrate, which has been proved by H NMR and mass spectra titration, scanning electronic microscope and dynamic light scattering analyses. More importantly, the quantification of citrate in artificial urine may develop TPE-Py fluorometric probe for the citrate detection in real biosystems.
柠檬酸盐是柠檬酸循环中的重要中间产物，是动物、植物和细菌的重要代谢途径。检测人体中柠檬酸盐的水平非常重要，因为几种疾病可能导致柠檬酸盐异常。在本文中，我们合成了一种新的基于经典四苯基乙烯（TPE）的聚集诱导发射（AIE）荧光团和基于联吡啶的酰胺的作为识别受体的荧光传感器（TPE-Py），用于检测柠檬酸盐。探针对柠檬酸盐具有良好的选择性和灵敏度，检测限相对较低（1.0×10^-6M）。荧光增强与基于 TPE-Py 与柠檬酸盐之间的氢键和静电相互作用的 AIE 特性有关，这已通过核磁共振和质谱滴定、扫描电子显微镜和动态光散射分析得到证明。更重要的是，人工尿液中柠檬酸盐的定量可能会开发 TPE-Py 荧光探针，用于实际生物体系中柠檬酸盐的检测。
