College of Chemistry and Materials, Fujian Normal University, Fuzhou 350007, P. R. China.
Analyst. 2019 Apr 8;144(8):2662-2669. doi: 10.1039/c9an00161a.
Aggregation-induced emission (AIE) provides a new strategy for preparing fluorescent sensors in aggregated state. In this paper, a series of crown ether-bridged bis-tetraphenylethylene compounds were synthesized in 78-84% yield by a simple procedure. The molecules exhibited excellent AIE properties in THF/H2O solutions and solid films. The investigation on sensing abilities for various biomolecules and metal ions suggested that Bis-TPE-1 possessed obvious response to folic acid, with fluorescence enhancement and blue shift of maximum emission wavelength from 380 nm to 365 nm. The detection limit for folic acid was 6.36 × 10-7 M, and the sensor's selectivity for folic acid was little interfered by the other species. The sensor mechanism was studied by FT-IR, 1H NMR, MS spectra and fluorescence Jobs' plot. The selective sensor for folic acid was applied in test paper and the analyses of real samples of mung bean and spinach. The superior bioimaging performance of Bis-TPE-1 for sensing folic acid was confirmed by the live cell imaging experiments, which indicated its good practical application potential for detecting folic acid.
聚集诱导发光（AIE）为制备聚集态荧光传感器提供了新策略。本文通过简单的方法以 78-84%的产率合成了一系列冠醚桥联双四苯基乙烯化合物。这些分子在 THF/H2O 溶液和固体薄膜中表现出优异的 AIE 性质。对各种生物分子和金属离子的传感能力的研究表明，Bis-TPE-1 对叶酸具有明显的响应，最大发射波长从 380nm 蓝移至 365nm，荧光增强。叶酸的检测限为 6.36×10-7 M，传感器对叶酸的选择性几乎不受其他物质的干扰。通过傅里叶变换红外光谱（FT-IR）、1H 核磁共振谱（1H NMR）、质谱（MS）谱和荧光 Jobs 图研究了传感器的机制。该选择性传感器用于检测绿豆和菠菜的实际样品，并在试纸上进行了分析。通过活细胞成像实验证实了 Bis-TPE-1 对叶酸传感的优异生物成像性能，表明其在检测叶酸方面具有良好的实际应用潜力。
