School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China.
School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China.
Talanta. 2018 Feb 1;178:980-986. doi: 10.1016/j.talanta.2017.09.045. Epub 2017 Sep 19.
A reliable and effective HPLC analytical method has been developed to stereoselectively quantify metconazole in soil and flour matrices. Effects of polysaccharide chiral stationary phase, type and content of alcoholic modifier on separation of racemic metconazole have been discussed in detail. Resolution and quantitative determination of metconazole stereoisomers were performed by using an Enantiopak OD column, with the n-hexane-ethanol mixture (97:3, v/v) at the flow rate of 1.0mL/min. Then, extraction and cleanup procedures followed by the modified QuEChERS (quick, easy, cheap, effective, rugged and safe) method were used for metconazole racemate in soil and flour matrices. The residual analysis method was validated. Good linearity (R ≥ 0.9997) and recoveries (94.98-104.89%, RSD ≤ 2.0%) for four metconazole stereoisomers were obtained. In brief, this proposed method showed good accuracy and precision, which might be applied in enantioselective determination, residual quantitative analysis, and degradation of metconazole in food and environmental matrices.
已经开发出一种可靠有效的 HPLC 分析方法，用于立体选择性定量测定土壤和面粉基质中的咪康唑。详细讨论了多糖手性固定相、醇改性剂的类型和含量对映体咪康唑分离的影响。通过使用 Enantiopak OD 柱，在流速为 1.0mL/min 的情况下，使用正己烷-乙醇混合物（97:3，v/v），对咪康唑对映异构体进行分离和定量测定。然后，采用改良的 QuEChERS（快速、简单、廉价、有效、坚固和安全）方法进行土壤和面粉基质中咪康唑外消旋体的提取和净化。残留分析方法已得到验证。四种咪康唑对映异构体均具有良好的线性（R≥0.9997）和回收率（94.98-104.89%，RSD≤2.0%）。总之，该方法具有良好的准确性和精密度，可应用于咪康唑在食品和环境基质中的对映选择性测定、残留定量分析和降解。
