School of Chemistry, South China Normal University, Guangzhou 510006, PR China.
Guangzhou Research & Creativity Biotechnology Co. Ltd., Guangzhou 510663, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Sep 15;1126-1127:121740. doi: 10.1016/j.jchromb.2019.121740. Epub 2019 Jul 30.
A fast analytical method through supercritical fluid chromatography was developed for enantioselective determination of triadimefon in soil and apple samples. Effects of chiral stationary phases, co-solvents, column temperature, and back pressure on chiral separation of triadimefon were discussed in detail. Chiral stationary phases containing electron-donating units located at phenylcarbamate moieties showed better enanitonrecognition abilities in triadimefon than those with electron-withdrawing units. Then, the modified pretreatment procedure was applied in enantioselective analysis of triadimefon in two matrices. When using cellulose tris(3,5-dimethylphenylcarbamate)-coated silica gel as the chiral stationary phase and the CO-methanol (95/5, v/v) mixture as the mobile phase, the LOD and LOQ were 0.38 mg/kg and 2.00 mg/kg, respectively, much lower than the others chiral stationary phases. Good linearity (R = 0.9999) and recoveries (92.90-105.56%，RSD ≤ 1.52%) for them were achieved.
建立了一种通过超临界流体色谱快速分析的方法，用于对土壤和苹果样品中的三唑酮进行对映选择性测定。详细讨论了手性固定相、共溶剂、柱温和背压对三唑酮手性分离的影响。含有在苯甲酰氨基部分中具有供电子单元的手性固定相比具有吸电子单元的手性固定相具有更好的三唑酮对映体识别能力。然后，对两种基质中三唑酮的对映选择性分析采用了改进的预处理程序。当使用纤维素三（3,5-二甲基苯基氨基甲酸酯）-涂覆硅胶作为手性固定相，以 CO-甲醇（95/5，v/v）混合物作为流动相时，LOD 和 LOQ 分别为 0.38mg/kg 和 2.00mg/kg，均低于其他手性固定相。对映体具有良好的线性（R=0.9999）和回收率（92.90-105.56%，RSD≤1.52%）。
