Emergency Department, 5th Hospital of Dongguan City, Dongguan, P.R. China.
Hepatobiliary Surgery Department, 5th Hospital of Dongguan City, Dongguan, P.R. China.
Oncol Res. 2018 Mar 5;26(2):315-322. doi: 10.3727/096504017X15067856789781. Epub 2017 Oct 3.
The aberrant expression of miR-433 has been validated in some types of cancers. However, the expression profile and the biological function of miR-433 on colon cancer are still elusive. This study was designed to investigate the function of miR-433 on the proliferation and invasion of colon cancer cells. We detected the expression of miR-433 in colon cancer tissues, adjacent normal tissues, and cell lines. CCK8 and Transwell assays were performed to explore the impact of miR-433 on colon cancer cell proliferation and invasion. The luciferase reporter assay was applied to identify the direct target of miR-433. The results demonstrated that miR-433 was downregulated in colon cancer tissues and cell lines when compared with the control. Overexpression of miR-433 significantly suppressed the ability of colon cancer cell proliferation and invasion, whereas knockdown of miR-433 remarkably enhanced cell proliferation and invasion. Homeobox A1 (HOXA1) was identified as a target of miR-433, and it mediated the functions of miR-433 on colon cancer cells. To conclude, we revealed that miR-433 was downregulated in colon cancer, and it inhibited colon cancer cell proliferation and invasion by directly targeting HOXA1.
miR-433 的异常表达已在某些类型的癌症中得到验证。然而，miR-433 在结肠癌中的表达谱和生物学功能仍不清楚。本研究旨在探讨 miR-433 对结肠癌细胞增殖和侵袭的功能。我们检测了结肠癌组织、相邻正常组织和细胞系中 miR-433 的表达。CCK8 和 Transwell 检测用于探索 miR-433 对结肠癌细胞增殖和侵袭的影响。荧光素酶报告基因检测用于鉴定 miR-433 的直接靶标。结果表明，与对照相比，miR-433 在结肠癌组织和细胞系中下调。miR-433 的过表达显著抑制结肠癌细胞增殖和侵袭的能力，而 miR-433 的敲低则显著增强细胞增殖和侵袭的能力。同源盒 A1（HOXA1）被鉴定为 miR-433 的靶标，它介导了 miR-433 对结肠癌细胞的功能。总之，我们揭示了 miR-433 在结肠癌中下调，并通过直接靶向 HOXA1 抑制结肠癌细胞增殖和侵袭。
