Department of Chemistry, Bar-Ilan University, Ramat-Gan 5290002, Israel.
Department of Chemistry, Bar-Ilan University, Ramat-Gan 5290002, Israel.
Ultrason Sonochem. 2018 Mar;41:288-296. doi: 10.1016/j.ultsonch.2017.09.032. Epub 2017 Sep 20.
There is an urgent need for new materials with antimicrobial activity. Phenolic essential oil (EO) compounds with Generally Recognized As Safe (GRAS) status are attractive candidates, but they need suitable delivery systems to overcome specific drawbacks. Core-shell microspheres (MSs) of Bovine Serum Albumin (BSA) or Human Serum Albumin (HSA) encapsulating such active compounds in the oil phase are a delivery system that is novel in combination with phenolic EO compounds. Moreover, the EO compounds can also be assembled in an oil shell around a protein core by choosing an appropriate oil phase. A facile sonochemical fabrication method, which can be easily scaled-up, is developed with full characterization of the resulting EO-containing MSs by optical and electron microscopy. Bacterial growth experiments with E. coli including TEM of treated cells confirm antibacterial activity. In the case of carvacrol, the corresponding MSs are found to be both more bioactive and more stable than the free biocide.
迫切需要具有抗菌活性的新材料。具有一般公认安全 (GRAS) 地位的酚类精油 (EO) 化合物是很有吸引力的候选物，但它们需要合适的输送系统来克服特定的缺点。牛血清白蛋白 (BSA) 或人血清白蛋白 (HSA) 的核壳微球 (MS) 将此类活性化合物包封在油相中，是一种与酚类 EO 化合物结合的新型输送系统。此外，通过选择合适的油相，EO 化合物也可以在油壳中围绕蛋白质核心组装。通过光学和电子显微镜对所得含 EO 的 MS 进行充分表征，开发了一种简便的超声化学制造方法，该方法易于放大。用大肠杆菌进行细菌生长实验，包括处理细胞的 TEM，证实了抗菌活性。就香芹酚而言，相应的 MS 比游离杀生物剂更具生物活性和稳定性。
