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两种谷物蛋白（ABA 胁迫与成熟蛋白（ASR））中的结构无序与诱导折叠。

Structural disorder and induced folding within two cereal, ABA stress and ripening (ASR) proteins.

机构信息

Laboratoire de Protection et d'Amélioration des Plantes, Centre de Biotechnologie de Sfax (CBS), Sfax, Tunisia.

Aix-Marseille Univ, CNRS, Architecture et Fonction des Macromolécules Biologiques (AFMB), UMR 7257, Marseille, France.

出版信息

Sci Rep. 2017 Nov 14;7(1):15544. doi: 10.1038/s41598-017-15299-4.

DOI:10.1038/s41598-017-15299-4
PMID:29138428
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5686140/
Abstract

Abscisic acid (ABA), stress and ripening (ASR) proteins are plant-specific proteins involved in plant response to multiple abiotic stresses. We previously isolated the ASR genes and cDNAs from durum wheat (TtASR1) and barley (HvASR1). Here, we show that HvASR1 and TtASR1 are consistently predicted to be disordered and further confirm this experimentally. Addition of glycerol, which mimics dehydration, triggers a gain of structure in both proteins. Limited proteolysis showed that they are highly sensitive to protease degradation. Addition of 2,2,2-trifluoroethanol (TFE) however, results in a decreased susceptibility to proteolysis that is paralleled by a gain of structure. Mass spectrometry analyses (MS) led to the identification of a protein fragment resistant to proteolysis. Addition of zinc also induces a gain of structure and Hydrogen/Deuterium eXchange-Mass Spectrometry (HDX-MS) allowed identification of the region involved in the disorder-to-order transition. This study is the first reported experimental characterization of HvASR1 and TtASR1 proteins, and paves the way for future studies aimed at unveiling the functional impact of the structural transitions that these proteins undergo in the presence of zinc and at achieving atomic-resolution conformational ensemble description of these two plant intrinsically disordered proteins (IDPs).

摘要

脱落酸（ABA）、胁迫和成熟（ASR）蛋白是植物特有的蛋白质，参与植物对多种非生物胁迫的反应。我们之前从硬粒小麦（TtASR1）和大麦（HvASR1）中分离出 ASR 基因和 cDNA。在这里，我们表明 HvASR1 和 TtASR1 一直被预测为无序，并且进一步通过实验证实了这一点。添加甘油模拟脱水，会引发这两种蛋白质结构的增加。有限的蛋白水解表明它们对蛋白酶降解高度敏感。然而，添加 2,2,2-三氟乙醇（TFE）会导致对蛋白水解的敏感性降低，这与结构的增加是平行的。质谱分析（MS）导致鉴定出一个对蛋白水解有抗性的蛋白片段。添加锌也会诱导结构的增加，氢/氘交换-质谱（HDX-MS）允许鉴定参与无序到有序转变的区域。这项研究是对 HvASR1 和 TtASR1 蛋白进行的首次报道的实验表征，为未来的研究铺平了道路，这些研究旨在揭示这些蛋白质在锌存在下发生的结构转变的功能影响，并实现对这两种植物固有无序蛋白（IDP）的结构转变的原子分辨率构象集合描述。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/8be943c652ac/41598_2017_15299_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/9ca374c2280e/41598_2017_15299_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/63fbc3a08ad0/41598_2017_15299_Fig6_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/f3552cedf924/41598_2017_15299_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/b186235075a0/41598_2017_15299_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/7e556a9c72ec/41598_2017_15299_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/8be943c652ac/41598_2017_15299_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/9ca374c2280e/41598_2017_15299_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/2d16c8cd5dc6/41598_2017_15299_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/ff3dab6cb223/41598_2017_15299_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/45d12197bf58/41598_2017_15299_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/c7f40c07a092/41598_2017_15299_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/63fbc3a08ad0/41598_2017_15299_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/faa2ad152911/41598_2017_15299_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/f3552cedf924/41598_2017_15299_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/b186235075a0/41598_2017_15299_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/7e556a9c72ec/41598_2017_15299_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4cb/5686140/8be943c652ac/41598_2017_15299_Fig11_HTML.jpg

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Phys Chem Chem Phys. 2016 Oct 7;18(37):25806-16. doi: 10.1039/c6cp02272c. Epub 2016 Jun 3.
3
Biochem Biophys Rep. 2025 Feb 28;42:101963. doi: 10.1016/j.bbrep.2025.101963. eCollection 2025 Jun.
4
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6
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5
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