Department of Occupational and Environmental Health, School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan, Hubei, 430065, China.
Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan, Hubei, 430065, China.
Sci Rep. 2017 Nov 14;7(1):15502. doi: 10.1038/s41598-017-15570-8.
Chronic airway inflammation is recognized as an essential process in the pathogenesis of asthma. Cytokine profiles derived from immune and inflammation cells such as T-helper (Th) cells, eosinophilia and neutrophilia are not limited to the Th2 type in asthma. However, little is understood about associations between Th2-low inflammatory cytokine profiles and risk of asthma in adults. A case-control study of 910 adult asthma and 881 healthy controls was conducted. Inflammatory cytokines screening was undertaken by high-throughput protein microarray technology, and Th17-related inflammatory cytokines (IL17A, IL-9, adipsin and CCL11) were finally selected. Associations between these four cytokines and adult asthma risk were analyzed by multivariate logistic regression models. We observed that plasma IL-17A and IL-9 levels were significantly increased in asthmatics when compared with controls. However, the plasma expressions of adipsin and CCL11 in asthmatics were significantly lower than that in health controls. The adjusted ORs (95%CI) of association between IL-17A, IL-9, adipsin and CCL11 expressions and adult asthma were 3.08 (1.91, 4.97), 1.93 (1.41, 2.64), 10.02 (6.99, 14.37) and 3.29 (2.36, 4.59), respectively (all P < 0.0001). Our results suggested that elevated IL-17A and IL-9 expressions and decreased levels of adipsin and CCL11 were positively associated with adult asthma.
慢性气道炎症被认为是哮喘发病机制中的一个重要过程。免疫和炎症细胞（如 T 辅助细胞、嗜酸性粒细胞和中性粒细胞）产生的细胞因子谱不仅限于哮喘中的 Th2 型。然而，对于 Th2 低炎症细胞因子谱与成人哮喘风险之间的关联知之甚少。我们进行了一项病例对照研究，纳入了 910 名成年哮喘患者和 881 名健康对照者。通过高通量蛋白质微阵列技术进行炎症细胞因子筛选，并最终选择了 Th17 相关炎症细胞因子（IL17A、IL-9、脂联素和 CCL11）。通过多变量逻辑回归模型分析这四种细胞因子与成人哮喘风险之间的关联。我们观察到，与对照组相比，哮喘患者的血浆 IL-17A 和 IL-9 水平显著升高。然而，哮喘患者的血浆脂联素和 CCL11 表达水平显著低于健康对照组。IL-17A、IL-9、脂联素和 CCL11 表达与成人哮喘之间的调整 OR（95%CI）分别为 3.08（1.91，4.97）、1.93（1.41，2.64）、10.02（6.99，14.37）和 3.29（2.36，4.59）（均 P < 0.0001）。我们的结果表明，IL-17A 和 IL-9 表达升高以及脂联素和 CCL11 水平降低与成人哮喘呈正相关。
