Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, Lodz, Poland.
Department of Medical Biochemistry, Medical University of Lodz, Mazowiecka 6/8, Lodz, Poland.
Oxid Med Cell Longev. 2017;2017:2181942. doi: 10.1155/2017/2181942. Epub 2017 Sep 12.
Nitric oxide (NO) is one of the most important signal molecules, involved in both physiological and pathological processes. As a neurotransmitter in the central nervous system, NO regulates cerebral blood flow, neurogenesis, and synaptic plasticity. The aim of our study was to investigate the effect of the extremely low-frequency electromagnetic field (ELF-EMF) on generation and metabolism of NO, as a neurotransmitter, in the rehabilitation of poststroke patients. Forty-eight patients were divided into two groups: ELF-EMF and non-ELF-EMF. Both groups underwent the same 4-week rehabilitation program. Additionally, the ELF-EMF group was exposed to an extremely low-frequency electromagnetic field of 40 Hz, 7 mT, for 15 min/day. Levels of 3-nitrotyrosine, nitrate/nitrite, and TNF in plasma samples were measured, and NOS2 expression was determined in whole blood samples. Functional status was evaluated before and after a series of treatments, using the Activity Daily Living, Geriatric Depression Scale, and Mini-Mental State Examination. We observed that application of ELF-EMF significantly increased 3-nitrotyrosine and nitrate/nitrite levels, while expression of NOS2 was insignificantly decreased in both groups. The results also show that ELF-EMF treatments improved functional and mental status. We conclude that ELF-EMF therapy is capable of promoting recovery in poststroke patients.
一氧化氮（NO）是最重要的信号分子之一，参与生理和病理过程。作为中枢神经系统的神经递质，NO 调节脑血流、神经发生和突触可塑性。我们的研究目的是探讨极低频电磁场（ELF-EMF）对脑卒中后患者神经递质 NO 的生成和代谢的影响。48 名患者分为两组：ELF-EMF 组和非 ELF-EMF 组。两组均接受相同的 4 周康复计划。此外，ELF-EMF 组接受 40Hz、7mT 的极低频电磁场照射，每天 15 分钟。测量血浆样本中 3-硝基酪氨酸、硝酸盐/亚硝酸盐和 TNF 的水平，并测定全血样本中 NOS2 的表达。在一系列治疗前后，使用活动日常生活量表、老年抑郁量表和简易精神状态检查评估功能状态。我们观察到，ELF-EMF 的应用显著增加了 3-硝基酪氨酸和硝酸盐/亚硝酸盐的水平，而两组 NOS2 的表达均无明显下降。结果还表明，ELF-EMF 治疗改善了功能和精神状态。我们得出结论，ELF-EMF 治疗能够促进脑卒中后患者的康复。
