Jin Mei-Yu, Wang Miao, Wu Xiao-Han, Fan Ming-Zhi, Li Han-Xi, Guo Yu-Qing, Jiang Jun, Yin Cheng-Ri, Lian Mei-Lan
Department of Chemistry, Yanbian University, Park Road 977, Yanji 133002, China.
Agricultural College, Yanbian University, Park Road 977, Yanji 133002, China.
Plants (Basel). 2023 May 30;12(11):2174. doi: 10.3390/plants12112174.
is an endangered medicinal plant, and adventitious root (AR) culture is an effective way to obtain its raw materials. Yeast extract (YE) is a lower-price elicitor and can efficiently promote metabolite synthesis. In this study, the bioreactor-cultured ARs were treated with YE in a suspension culture system to investigate the elicitation effect of YE on flavonoid accumulation, serving for further industrial production. Among YE concentrations (25-250 mg/L), 100 mg/L YE was the most suitable for increasing the flavonoid accumulation. The ARs with various ages (35-, 40-, and 45-day-old) responded differently to YE stimulation, where the highest flavonoid accumulation was found when 35-day-old ARs were treated with 100 mg/L YE. After YE treatment, the flavonoid content increased, peaked at 4 days, and then decreased. By comparison, the flavonoid content and antioxidant activities in the YE group were obviously higher than those in the control. Subsequently, the flavonoids of ARs were extracted by flash extraction, where the optimized extraction process was: 63% ethanol, 69 s of extraction time, and a 57 mL/g liquid-material ratio. The findings provide a reference for the further industrial production of flavonoid-enriched ARs, and the cultured ARs have potential application for the future production of products.
是一种濒危药用植物，不定根（AR）培养是获取其原料的有效途径。酵母提取物（YE）是一种价格较低的诱导子，能有效促进代谢物合成。在本研究中，在悬浮培养系统中用YE处理生物反应器培养的不定根，以研究YE对黄酮类化合物积累的诱导作用，为进一步的工业化生产提供依据。在YE浓度（25 - 250 mg/L）中，100 mg/L YE最适合增加黄酮类化合物的积累。不同年龄（35天、40天和45天）的不定根对YE刺激的反应不同，其中用100 mg/L YE处理35天的不定根时黄酮类化合物积累最高。YE处理后，黄酮类化合物含量增加，在第4天达到峰值，然后下降。相比之下，YE组的黄酮类化合物含量和抗氧化活性明显高于对照组。随后，通过快速萃取法提取不定根中的黄酮类化合物，优化的萃取工艺为：63%乙醇、69 s萃取时间和57 mL/g液料比。这些研究结果为富含黄酮类化合物的不定根的进一步工业化生产提供了参考，并且培养的不定根在未来产品生产中具有潜在应用价值。
