Xu Yi, Lu Yan, Wu Hai-Ping, Bu Ying
China Pharmaceutical University, Nanjing 210009, China.
Institute for Drug and Instrument Control of Joint Logistics Department of Nanjing Military Area Command, Nanjing 210008, China.
Zhongguo Zhong Yao Za Zhi. 2017 Aug;42(15):2930-2933. doi: 10.19540/j.cnki.cjcmm.20170714.006.
Snake drugs have high values in clinical medication for anti-inflammatory, analgesia activities and dredging collaterals. However, owing to their deficient resource and substantial profit, many counterfeits for snake drugs have appeared on the market. Traditional methods for Chinese medicine authentication include identification of origin, morphology identification, microscopy and physiochemical identification. But these methods are restricted in application because of their high morphological requirement for specimens, complex process for assays and indeterminate results guided by subjective. With the development of molecular biology and molecular genetic techniques, new theories and technologies for molecular detection have been introduced to the authentication of Chinese medicine, such as RAPD, specific PCR amplification, DNA barcoding analysis and so on, improved the authentication system of Chinese medicine. Here, we will give a brief review of molecular detection methods for snake drugs authentication.
蛇类药材在临床用药中具有抗炎、镇痛和通络等很高的价值。然而，由于其资源匮乏且利润丰厚，市场上出现了许多蛇类药材的假冒品。中药鉴定的传统方法包括产地鉴定、形态鉴定、显微鉴定和理化鉴定。但这些方法在应用中受到限制，因为它们对标本的形态要求较高、检测过程复杂且结果受主观因素影响具有不确定性。随着分子生物学和分子遗传技术的发展，分子检测的新理论和技术被引入到中药鉴定中，如随机扩增多态性DNA（RAPD）、特异性PCR扩增、DNA条形码分析等，完善了中药鉴定体系。在此，我们将对蛇类药材鉴定的分子检测方法进行简要综述。
