Chen Shilin, Guo Baolin, Zhang Guijun, Yan Zhuyun, Luo Guangming, Sun Suqin, Wu Hezhen, Huang Linfang, Pang Xiaohui, Chen Jianbo
The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Zhongguo Zhong Yao Za Zhi. 2012 Apr;37(8):1043-55.
In this review, the authors summarized the new technologies and methods for identifying traditional Chinese medicinal materials, including molecular identification, chemical identification, morphological identification, microscopic identification and identification based on biological effects. The authors introduced the principle, characteristics, application and prospect on each new technology or method and compared their advantages and disadvantages. In general, new methods make the result more objective and accurate. DNA barcoding technique and spectroscopy identification have their owner obvious strongpoint in universality and digitalization. In the near future, the two techniques are promising to be the main trend for identifying traditional Chinese medicinal materials. The identification techniques based on microscopy, liquid chromatography, PCR, biological effects and DNA chip will be indispensable supplements. However, the bionic identification technology is just placed in the developing stage at present.
在本综述中，作者总结了鉴定中药材的新技术和方法，包括分子鉴定、化学鉴定、形态鉴定、显微鉴定以及基于生物学效应的鉴定。作者介绍了每种新技术或方法的原理、特点、应用及前景，并比较了它们的优缺点。总体而言，新方法使结果更加客观准确。DNA条形码技术和光谱鉴定在通用性和数字化方面各有明显优势。在不久的将来，这两种技术有望成为鉴定中药材的主要趋势。基于显微镜、液相色谱、聚合酶链反应、生物学效应和DNA芯片的鉴定技术将是不可或缺的补充。然而，仿生鉴定技术目前尚处于发展阶段。
