Wang Haolu, Thorling Camilla A, Xu Zhi Ping, Crawford Darrell H G, Liang Xiaowen, Liu Xin, Roberts Michael S
Therapeutics Research Centre, School of Medicine, The University of Queensland, Princess Alexandra Hospital, Woolloongabba, Australia.
Department of Biliary-Pancreatic Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Curr Protoc Stem Cell Biol. 2017 Nov 15;43:2B.8.1-2B.8.17. doi: 10.1002/cpsc.39.
This unit describes a protocol for elucidating the in vivo disposition of administered mesenchymal stem cells (MSCs). Specifically, direct visualization of donor cell spatiotemporal distribution and assessment of donor cell quantity in recipient organs are described. Protocols for data analysis are suggested, with the goal of developing a model to characterize and predict the physiological kinetics of administered MSCs. The use of this model is described, suggesting that it can be applied to abnormal conditions and has potential interspecies and inter-route predictive capability. These universal methods can be employed, regardless of the type of stem cell or disease, to guide future experiments and design treatment protocols. © 2017 by John Wiley & Sons, Inc.
本单元描述了一种用于阐明所施用间充质干细胞（MSC）体内处置情况的方案。具体而言，描述了供体细胞时空分布的直接可视化以及受体器官中供体细胞数量的评估。建议了数据分析方案，目标是建立一个模型来表征和预测所施用MSC的生理动力学。描述了该模型的用途，表明它可应用于异常情况，并具有潜在的种间和不同给药途径的预测能力。无论干细胞类型或疾病如何，均可采用这些通用方法来指导未来实验和设计治疗方案。© 2017约翰威立国际出版公司
