1 LSUHSC SOM's Department of Surgery, Louisiana State University Health Sciences Center School of Medicine , New Orleans, Louisiana.
2 New Orleans BioInnovation Center , LaCell LLC, New Orleans Louisiana.
Tissue Eng Part C Methods. 2018 Mar;24(3):135-145. doi: 10.1089/ten.TEC.2017.0339. Epub 2018 Jan 9.
White adipose tissue (WAT) is a critical organ in both health and disease. However, physiologically faithful tissue culture models of primary human WAT remain limited, at best. In this study we describe a novel WAT culture system in which primary human WAT is sandwiched between tissue-engineered sheets of adipose-derived stromal cells. This construct, called "sandwiched white adipose tissue" (SWAT), can be defined as a microphysiological system (MPS) since it is a tissue-engineered, multicellular, three-dimensional organ construct produced using human cells. We validated SWAT against the National Institutes of Health MPS standards and found that SWAT is viable in culture for 8 weeks, retains physiologic responses to exogenous signaling, secretes adipokines, and engrafts into animal models. These attributes position SWAT as a powerful tool for the study of WAT physiology, pathophysiology, personalized medicine, and pharmaceutical development.
白色脂肪组织（WAT）在健康和疾病中都是一个关键器官。然而，生理上忠实的原代人 WAT 组织培养模型仍然非常有限。在这项研究中，我们描述了一种新型的 WAT 培养系统，其中原代人 WAT 被夹在脂肪衍生基质细胞的组织工程片之间。这种构建体被称为“夹心白色脂肪组织”（SWAT），可以被定义为一个微生理系统（MPS），因为它是使用人体细胞制造的组织工程、多细胞、三维器官构建体。我们针对 NIH MPS 标准对 SWAT 进行了验证，发现 SWAT 在培养中可存活 8 周，对外源信号仍具有生理反应，分泌脂肪因子，并能在动物模型中植入。这些特性使 SWAT 成为研究 WAT 生理学、病理生理学、个性化医学和药物开发的有力工具。
