Chen Chen, Li Xin-Na, Li Gui-Xia, Zhao Li, Duan Su-Xia, Yan Teng-Fei, Feng Zhi-Shan, Ma Xue-Jun
Key Laboratory for Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
Pediatric Research Institute, Children's Hospital of Hebei Province, Shijiazhuang, 050031, Hebei Province, China.
Diagn Microbiol Infect Dis. 2018 Feb;90(2):90-95. doi: 10.1016/j.diagmicrobio.2017.10.005. Epub 2017 Oct 14.
In this study, a rapid reverse-transcription recombinase aided amplification (RT-RAA) assay was developed to detect respiratory syncytial virus (RSV) subgroups A and B, respectively. The reaction was performed at 39°C in less than 30min. The analytical sensitivities of RSVA and RSVB at 95% probability by probit regression analysis were 38copies per reaction and 35 copies per reaction, respectively, and no cross reactions with other related respiratory viruses were observed. The RT-RAA assay was further utilized to detect and subgroup 306 clinical specimens and the results showed that 79(25.82%, 79/306) samples were positive for RSV, of those 16(20.25%, 16/79) were identified as RSVA and 63(79.75%, 63/79) were RSVB, which is completely consistent with the results obtained by RSV RT-qPCR assay. In conclusion, the developed RAA assay will be of benefit as a faster, sensitive and specific alternative tool for detection of RSV.
在本研究中，开发了一种快速逆转录重组酶辅助扩增（RT-RAA）检测方法，分别用于检测呼吸道合胞病毒（RSV）A、B亚组。反应在39℃下进行，耗时不到30分钟。通过概率回归分析，RSVA和RSVB在95%概率下的分析灵敏度分别为每个反应38拷贝和35拷贝，且未观察到与其他相关呼吸道病毒的交叉反应。进一步利用RT-RAA检测方法对306份临床标本进行检测和分型，结果显示79份（25.82%，79/306）样本RSV呈阳性，其中16份（20.25%，16/79）被鉴定为RSVA，63份（79.75%，63/79）为RSVB，这与RSV RT-qPCR检测方法获得的结果完全一致。总之，所开发的RAA检测方法作为一种更快、灵敏且特异的RSV检测替代工具将具有重要价值。
