Pharmacognosy Division, CSIR-National Botanical Research Institute, Lucknow, U.P, 226001, India.
Pharmacognosy Division, CSIR-National Botanical Research Institute, Lucknow, U.P, 226001, India.
J Ethnopharmacol. 2020 Mar 1;249:112387. doi: 10.1016/j.jep.2019.112387. Epub 2019 Nov 20.
Gloriosa superba L. (Colchicaceae) is used in the treatment of gout and rheumatism as a traditional medicine dates back to 1810. It has also been used as ethnobotanical and folklore medicine to induce abortion/vaginal poison.
The present study was carried out to identify the chemical variation existing in the major alkaloid metabolite (colchicine) in a threatened species, Gloriosa superba L. and is correlated with invitro antigout activity.
The samples (tuber) were collected from their natural locations in Gangetic plain of India. HPLC-PDA quantification of colchicine was done on C column at 245 nm and invitro antigout activity was analyzed by inhibition of protein denaturation, DPPH and Hydroxyl radical scavenging assay.
The colchicine content within the 29 samples ranges from 0.021 to 0.665% and the maximum contents was in NBG-10 from Kanth (U.P). Such high colchicine (0.665%) containing natural population of G. superba is reported for the first time in Indian population. Four chemotypes viz. NBG-10, NBG-120, NBG-126 and NBG-88 were selected on the basis of colchicine content for invitro antigout activity. NBG-10 was separated from rest of the population exhibiting the most promising activity with high colchicine content.
The outcomes will be helpful in the identification of elite chemotype for herbal product development and quality check of metabolites in raw material. The study will also support the site-specific commercial cultivation to meet out the industrial demand as well as income generation to farmers.
Gloriosa superba L.（秋水仙科）作为一种传统药物，自 1810 年以来就被用于治疗痛风和风湿病。它也被用作民族植物学和民间医学，用于堕胎/阴道中毒。
本研究旨在鉴定受威胁物种Gloriosa superba L. 中主要生物碱代谢物（秋水仙碱）的化学变异，并与体外抗痛风活性相关。
样品（块茎）从印度恒河平原的自然栖息地采集。在 245nm 处使用 C 柱通过 HPLC-PDA 对秋水仙碱进行定量，并通过抑制蛋白质变性、DPPH 和羟基自由基清除测定分析体外抗痛风活性。
29 个样本中的秋水仙碱含量范围为 0.021-0.665%，含量最高的是坎特的 NBG-10（北方邦）。印度人口中首次报道了含有如此高含量秋水仙碱（0.665%）的天然 G. superba 种群。根据秋水仙碱含量，选择了四个化学型，即 NBG-10、NBG-120、NBG-126 和 NBG-88，用于体外抗痛风活性。NBG-10 与其余种群分离，表现出最高的活性和高含量的秋水仙碱。
这些结果将有助于鉴定草药产品开发的优良化学型，并检查原料中代谢物的质量。该研究还将支持特定地点的商业种植，以满足工业需求并为农民创造收入。
