Singh Tripti, Srivastava Neha, Bhatiya A K, Mishra P K
Department of Biotechnology, GLA University, Mathura, U.P. 281406 India.
Department of Chemical Engineering and Technology, IIT (BHU), Varanasi, U.P. 221005 India.
3 Biotech. 2017 Dec;7(6):384. doi: 10.1007/s13205-017-1006-0. Epub 2017 Oct 31.
This study evaluated the capability of ABHI001 to effectively degrade -cresol through different techniques. The molecular identity of the laboratory isolate ABHI001 was confirmed through the 16S ribosomal DNA gene pattern, and its morphological features were investigated through field-emission scanning electron microscopy. In addition, the degradation behavior of the isolate for cresol was verified using several techniques, including UV-visible spectroscopy, followed by high-performance liquid chromatography (HPLC), gas chromatography, and Fourier transform infrared spectroscopy. The maximum degradation percentage of 85% for -cresol could be achieved after 18 h of incubation with . ABHI001. The formation of -hydroxybenzaldehyde, -hydroxybenzoate, and protocatechuate metabolites was confirmed through HPLC. The study results indicate that . ABHI001 may have applications in the bioremediation of organic pollutants, such as -cresol.
本研究评估了ABHI001通过不同技术有效降解对甲酚的能力。通过16S核糖体DNA基因图谱确认了实验室分离株ABHI001的分子身份，并通过场发射扫描电子显微镜研究了其形态特征。此外，使用多种技术验证了该分离株对甲酚的降解行为，包括紫外可见光谱，随后是高效液相色谱（HPLC）、气相色谱和傅里叶变换红外光谱。与ABHI001孵育18小时后，对甲酚的最大降解率可达85%。通过HPLC确认了对羟基苯甲醛、对羟基苯甲酸和原儿茶酸代谢物的形成。研究结果表明，ABHI001可能在有机污染物如对甲酚的生物修复中具有应用价值。
