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银纳米颗粒-人血红蛋白界面：冠层形成和相互作用现象的时间演变

Silver nanoparticle-human hemoglobin interface: time evolution of the corona formation and interaction phenomenon.

作者信息

Bhunia A K, Kamilya T, Saha S

机构信息

Department of Physics & Technophysics, Vidyasagar University, Paschim Medinipur, 721102 India.

Department of Physics, Government General Degree College at Gopiballavpur-II, Beliaberah Jhargram, 721517 India.

出版信息

Nano Converg. 2017;4(1):28. doi: 10.1186/s40580-017-0122-1. Epub 2017 Oct 30.

DOI:10.1186/s40580-017-0122-1
PMID:29142807
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5661023/
Abstract

In this paper, we have used spectroscopic and electron microscopic analysis to monitor the time evolution of the silver nanoparticles (Ag NP)-human hemoglobin (Hb) corona formation and to characterize the interaction of the Ag NPs with Hb. The time constants for surface plasmon resonance binding and reorganization are found to be 9.51 and 118.48 min, respectively. The drop of surface charge and the increase of the hydrodynamic diameter indicated the corona of Hb on the Ag NP surface. The auto correlation function is found to broaden with the increasing time of the corona formation. Surface zeta potential revealed that positively charged Hb interact electrostatically with negatively charged Ag NP surfaces. The change in α helix and β sheet depends on the corona formation time. The visualization of the Hb corona from HRTEM showed large number of Hb domains aggregate containing essentially Ag NPs and without Ag NPs. Emission study showed the tertiary deformation, energy transfer, nature of interaction and quenching under three different temperatures.

摘要

在本文中，我们利用光谱和电子显微镜分析来监测银纳米颗粒（Ag NP）-人血红蛋白（Hb）冠层形成的时间演变，并表征Ag NPs与Hb的相互作用。发现表面等离子体共振结合和重组的时间常数分别为9.51和118.48分钟。表面电荷的下降和流体动力学直径的增加表明了Ag NP表面上Hb冠层的形成。发现自相关函数随着冠层形成时间的增加而变宽。表面zeta电位表明带正电的Hb与带负电的Ag NP表面发生静电相互作用。α螺旋和β折叠的变化取决于冠层形成时间。高分辨率透射电子显微镜（HRTEM）对Hb冠层的观察显示，大量Hb结构域聚集，其中一些含有Ag NPs，一些不含Ag NPs。发射研究表明了在三种不同温度下的三级变形、能量转移、相互作用性质和猝灭。

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J Colloid Interface Sci. 2017 Nov 1;505:1165-1171. doi: 10.1016/j.jcis.2017.07.011. Epub 2017 Jul 5.
2
Size-dependent interaction of silica nanoparticles with lysozyme and bovine serum albumin proteins.尺寸依赖的二氧化硅纳米颗粒与溶菌酶和牛血清白蛋白蛋白质的相互作用。
Phys Rev E. 2016 May;93(5):052601. doi: 10.1103/PhysRevE.93.052601. Epub 2016 May 2.
3
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Front Chem. 2020 Aug 11;8:703. doi: 10.3389/fchem.2020.00703. eCollection 2020.
4
Regulation of cellular gene expression by nanomaterials.纳米材料对细胞基因表达的调控
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5
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Environ Sci Pollut Res Int. 2018 Nov;25(32):32373-32380. doi: 10.1007/s11356-018-3217-2. Epub 2018 Sep 18.
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J Phys Chem B. 2015 Jul 30;119(30):9461-76. doi: 10.1021/acs.jpcb.5b00436. Epub 2015 Jul 13.
4
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Photoacoustics. 2015 Jan 13;3(1):26-34. doi: 10.1016/j.pacs.2014.12.003. eCollection 2015 Mar.
5
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6
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