Department of Environmental Engineering, Harbin Institute of Technology, Weihai, 2# Wenhua West Road, Weihai, 264209, People's Republic of China.
Guangzhou Key Laboratory of Environmental Exposure and Health, School of Environment, Jinan University, Guangzhou, 510632, People's Republic of China.
Environ Sci Pollut Res Int. 2018 Nov;25(32):32373-32380. doi: 10.1007/s11356-018-3217-2. Epub 2018 Sep 18.
This work reports the toxicity of small silver nanoparticles (nanoAg, 20 nm) and silver ions (Ag) to the red blood cells with the silver concentration level of 10 g/mL. Results show that red blood cells (RBCs) start hemolysis when treated by nanoAg of 1.5 × 10 g/mL or Ag of 2.9 × 10 g/mL. A low ATPase activity of 30% has been observed after RBCs being treated with Ag of 2.6 × 10 g/mL, while the nanoAg does not obviously affect the ATPase activity. In molecular level, Ag is more toxic to the amino acid residues than nanoAg according to the change of fluorescence characteristics of hemoglobin (Hb). However, the nanoAg has been found to be more toxic than Ag to the secondary structure of Hb in terms of the loss of α-helix content.
这项工作报道了银纳米粒子（nanoAg，20nm）和银离子（Ag）在银浓度为 10μg/mL 时对红细胞的毒性。结果表明，当红细胞（RBCs）受到 1.5×10μg/mL 的 nanoAg 或 2.9×10μg/mL 的 Ag 的处理时，开始发生溶血。当 RBCs 受到 2.6×10μg/mL 的 Ag 处理时，ATP 酶活性已经观察到低至 30%，而 nanoAg 对 ATP 酶活性没有明显影响。在分子水平上，根据血红蛋白（Hb）荧光特性的变化，Ag 对氨基酸残基的毒性大于 nanoAg。然而，就 Hb 二级结构中α-螺旋含量的损失而言，nanoAg 被发现比 Ag 对 Hb 更具毒性。
