带正电荷的咪唑基离子液体保护的银纳米颗粒：根管治疗中有前景的消毒剂。
Positively charged imidazolium-based ionic liquid-protected silver nanoparticles: a promising disinfectant in root canal treatment.
作者信息
Abbaszadegan A, Nabavizadeh M, Gholami A, Aleyasin Z S, Dorostkar S, Saliminasab M, Ghasemi Y, Hemmateenejad B, Sharghi H
机构信息
Department of Endodontics, Faculty of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Pharmaceutical Biotechnology and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
Int Endod J. 2015 Aug;48(8):790-800. doi: 10.1111/iej.12377. Epub 2014 Oct 1.
AIM
To synthesize and characterize silver nanoparticles (Ag NPs) with different surface charges in order to evaluate their cytotoxicity and antibacterial activity in the absence and presence of dentine compared with NaOCl and CHX.
METHODOLOGY
Ag NPs with positive, negative and neutral surface charges were synthesized and characterized. The first phase of the experiment determined the minimum inhibitory concentrations (MICs) of NPs against planktonic E. faecalis and compared them with that of NaOCl and CHX. The second phase tested the elimination of E. faecalis at different contact times (5, 20 and 60 min and 4 and 24 h), and the role of dentine in their inactivation was assessed. In the third phase, the most effective Ag NP solution was selected for cytocompatibility assessment. An MTT-based cytotoxicity assay was used to evaluate the cytotoxicity of the selected NP solution in different concentrations on L929 fibroblasts compared to that of 2.5% NaOCl and 0.2% CHX. Student's t-test and repeated measures manova approach were used for statistical analyses.
RESULTS
The characterization revealed synthesis of colloidal NPs in the size range of 5-10 nm in diameter. The results indicated that Ag NP with a positive surface charge had the smallest MIC against planktonic E. faecalis, and it was active in very lower concentrations compared to NaOCl, CHX and the other tested AgNPs. Positive-charged Ag NPs at 5.7 × 10(-10) mol L(-1) completely prevented the growth of E. faecalis after 5 min of contact time, a finding comparable to 0.025% NaOCl. Dentine powder had variable inhibitory effects on all tested materials after 1 h incubation period, but after 24 h, NaOCl and the positive-charged Ag NPs were not inhibited by dentine at any concentration used. CHX was the most and the positively charged Ag NP solution was the least toxic solutions to L929 fibroblasts (P < 0.001).
CONCLUSIONS
Ag NP surface charge was important in bactericidal efficacy against E. faecalis. The positively charged imidazolium-based ionic liquid-protected Ag NPs showed promising antibacterial results against E. faecalis and exhibited a high level of cytocompatibility to L929 cells.
目的
合成并表征具有不同表面电荷的银纳米颗粒（Ag NPs），以评估其在有无牙本质存在的情况下与次氯酸钠（NaOCl）和氯己定（CHX）相比的细胞毒性和抗菌活性。
方法
合成并表征了具有正、负和中性表面电荷的Ag NPs。实验的第一阶段确定了纳米颗粒对浮游粪肠球菌的最低抑菌浓度（MICs），并将其与NaOCl和CHX的最低抑菌浓度进行比较。第二阶段测试了在不同接触时间（5、20和60分钟以及4和24小时）对粪肠球菌的清除情况，并评估了牙本质在其失活过程中的作用。在第三阶段，选择最有效的Ag NP溶液进行细胞相容性评估。与2.5% NaOCl和0.2% CHX相比，使用基于MTT的细胞毒性试验来评估所选纳米颗粒溶液在不同浓度下对L929成纤维细胞的细胞毒性。采用学生t检验和重复测量方差分析方法进行统计分析。
结果
表征显示合成的胶体纳米颗粒直径在5-10纳米范围内。结果表明，具有正表面电荷的Ag NP对浮游粪肠球菌的MIC最小，与NaOCl、CHX和其他测试的Ag NPs相比，它在非常低的浓度下就具有活性。在接触时间为5分钟后，浓度为5.7×10⁻¹⁰ mol L⁻¹的带正电荷的Ag NPs完全抑制了粪肠球菌的生长，这一结果与0.025% NaOCl相当。在孵育1小时后，牙本质粉末对所有测试材料具有不同的抑制作用，但在24小时后，NaOCl和带正电荷的Ag NPs在任何使用浓度下均未受到牙本质的抑制。CHX对L929成纤维细胞的毒性最大，带正电荷的Ag NP溶液毒性最小（P < 0.001）。
结论
Ag NP表面电荷对粪肠球菌的杀菌效果很重要。带正电荷的基于咪唑鎓离子液体保护的Ag NPs对粪肠球菌显示出有前景的抗菌结果，并且对L929细胞表现出高度的细胞相容性。
Zotero 插件