Karimi Sediqe, Mirhendi Hossein, Zaniani Fatemh Riyahi, Manesh Soroor Erfani, Salehi Mahshd, Esfahani Bahram Nasr
Department of Microbiology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Medical Parasitology and Mycology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2017 Oct 16;6:126. doi: 10.4103/abr.abr_240_16. eCollection 2017.
Molecular methods for the detection of drug-resistant tuberculosis (DR-TB) are potentially more rapid than conventional culture-based drug susceptibility testing, facilitating the commencement of appropriate treatment for patients with DR-TB. The aim of this study was to evaluate and develop polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays for the detection of mutations within , and for the determination of streptomycin (STR) resistance in .
Clinical specimens were collected from individuals with suspected TB referred to the TB Center of Isfahan, from which 205 were isolated and identified by conventional phenotypic methods. The minimum inhibitory concentration of STR for all isolates was determined using the proportion method and 10 isolates were recognized as STR resistant . The effect of genetic alterations in the gene for these resistant isolates were investigated by PCR-RFLP method.
Three (30%) isolates showed point mutation at codon 43 by RLFP analysis.
Our results suggest that RFLP analysis of the gene is useful for the rapid prediction of STR resistant strains of .
检测耐多药结核病（DR-TB）的分子方法可能比传统的基于培养的药敏试验更快，有助于为DR-TB患者开始适当的治疗。本研究的目的是评估和开发聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析方法，用于检测[具体基因名称未给出]内的突变，并确定[具体菌种未给出]对链霉素（STR）的耐药性。
从转诊至伊斯法罕结核病中心的疑似结核病患者中收集临床标本，通过传统表型方法分离并鉴定出205株[具体菌种未给出]。使用比例法测定所有分离株对STR的最低抑菌浓度，10株分离株被鉴定为对STR耐药。通过PCR-RFLP方法研究这些耐药分离株[具体基因名称未给出]基因中的遗传改变的影响。
通过RLFP分析，3株（30%）分离株在密码子43处出现点突变。
我们的结果表明，对[具体基因名称未给出]基因进行RFLP分析有助于快速预测[具体菌种未给出]的STR耐药菌株。
