Unni L K, Hannant M E, Becker R E, Giacobini E
Department of Psychiatry, Southern Illinois University School of Medicine, Springfield 62794-9230.
Clin Chem. 1989 Feb;35(2):292-5.
Physostigmine (Phy) was determined in plasma and cerebrospinal fluid (CSF) by HPLC with electrochemical detection, with use of a normal-phase column and methanolic sodium acetate buffer, pH 4.6. The detection limit of the method was 0.5 micrograms/L for a 2-mL sample of plasma or 0.5 mL of CSF. Analytical recovery of Phy in the range from 0.5 to 40 micrograms/L was 60% (SD 5%) for plasma and 78% (SD 8%) for CSF. Excellent chromatographic separation of Phy without column deterioration during extended usage and constant recovery for a wide range of Phy concentrations makes the routine monitoring of plasma from patients with Alzheimer's disease economically feasible. Using our method, we measured Phy in 13 such patients' plasmas at 105 and 135 min after a 135-min intravenous infusion of 300, 600, and 900 micrograms of Phy per square meter of body surface. Mean values significantly increased with dose (P = 0.001), but differences between 105 and 135 min (P = 0.229) or between dose and time (P = 0.949) were not significant.
采用高效液相色谱-电化学检测法，使用正相柱和pH值为4.6的甲醇醋酸钠缓冲液，测定血浆和脑脊液中的毒扁豆碱（Phy）。该方法对2 mL血浆样本或0.5 mL脑脊液样本的检测限为0.5微克/升。血浆中Phy在0.5至40微克/升范围内的分析回收率为60%（标准差5%），脑脊液中为78%（标准差8%）。Phy具有出色的色谱分离效果，在长期使用过程中柱性能不下降，且在宽浓度范围内回收率恒定，这使得对阿尔茨海默病患者血浆进行常规监测在经济上可行。使用我们的方法，在每平方米体表面积静脉输注300、600和900微克Phy 135分钟后，于105分钟和135分钟测定了13例此类患者血浆中的Phy。平均值随剂量显著增加（P = 0.001），但105分钟和135分钟之间的差异（P = 0.229）或剂量与时间之间的差异（P = 0.949）不显著。
