Department of Biochemistry and Cambridge Systems Biology Centre, University of Cambridge, UK.
Department of Biochemistry and Cambridge Systems Biology Centre, University of Cambridge, UK.
Curr Opin Chem Biol. 2018 Feb;42:42-50. doi: 10.1016/j.cbpa.2017.10.018. Epub 2017 Nov 13.
Lipids are a large and highly diverse family of biomolecules, which play essential structural, storage and signalling roles in cells and tissues. Although traditional mass spectrometry (MS) approaches used in lipidomics are highly sensitive and selective, lipid analysis remains challenging due to the chemical diversity of lipid structures, multiple isobaric species and incomplete separation using many forms of chromatography. Ion mobility (IM) separates ions in the gas phase based on their physicochemical properties. Addition of IM to the traditional lipidomic workflow both enhances separation of complex lipid mixtures, beneficial for lipid identification, and improves isomer resolution. Herein, we discuss the recent developments in IM-MS for lipidomics.
脂质是一大类具有高度多样性的生物分子，它们在细胞和组织中发挥着重要的结构、储存和信号作用。尽管脂质组学中使用的传统质谱 (MS) 方法具有很高的灵敏度和选择性，但由于脂质结构的化学多样性、多种等质异位素物种以及许多形式的色谱法不完全分离，脂质分析仍然具有挑战性。离子淌度 (IM) 根据离子的物理化学性质在气相中对离子进行分离。在传统的脂质组学工作流程中加入 IM 不仅增强了复杂脂质混合物的分离，有利于脂质的鉴定，而且提高了异构体的分辨率。本文讨论了 IM-MS 在脂质组学中的最新进展。
