基于结构选择性离子淌度-质谱的高置信度 shotgun 脂质组学。
High Confidence Shotgun Lipidomics Using Structurally Selective Ion Mobility-Mass Spectrometry.
机构信息
Center for Innovative Technology, Department of Chemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt-Ingram Cancer Center, Vanderbilt University, Nashville, TN, USA.
Department of Chemistry, Vanderbilt University, Nashville, TN, USA.
出版信息
Methods Mol Biol. 2021;2306:11-37. doi: 10.1007/978-1-0716-1410-5_2.
Abstract
Ion mobility (IM) is a gas phase separation strategy that can either supplement or serve as a high-throughput alternative to liquid chromatography (LC) in shotgun lipidomics. Incorporating the IM dimension in untargeted lipidomics workflows can help resolve isomeric lipids, and the collision cross section (CCS) values obtained from the IM measurements can provide an additional molecular descriptor to increase lipid identification confidence. This chapter provides a broad overview of an untargeted ion mobility-mass spectrometry (IM-MS) workflow using a commercial drift tube ion mobility-quadrupole-time-of-flight mass spectrometer (IM-QTOF) for high confidence lipidomics.
摘要
离子淌度（IM）是一种气相分离策略，在 shotgun 脂质组学中，它既可以作为液相色谱（LC）的补充，也可以作为其高通量替代方法。在非靶向脂质组学工作流程中纳入 IM 维度可以帮助解析同分异构脂质，并且从 IM 测量中获得的碰撞截面（CCS）值可以提供附加的分子描述符，以提高脂质鉴定的可信度。本章提供了使用商用漂移管离子淌度-四极杆-飞行时间质谱仪（IM-QTOF）进行高可信度脂质组学的非靶向离子淌度-质谱（IM-MS）工作流程的概述。
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