Melani C, Canepa L, Sessarego M, Miglino M, Ferraris A M, Gaetani G F
Cattedra di Ematologia, Università di Genova, Italy.
Eur J Haematol. 1989 Jan;42(1):32-7. doi: 10.1111/j.1600-0609.1989.tb00243.x.
We describe here a patient with Ph'-positive chronic myelogenous leukemia (CML) who developed a Ph'-negative blastic crisis. The blast DNA was analyzed on two different occasions, at the beginning of the blastic phase and at the end, shortly before the patient's death. Although cells from both samples had no Ph' chromosome marker (not even a masked one) we could detect a rearrangement of the bcr gene in the second DNA sample, using a '3'-bcr' probe. The same probe and a '5'-bcr' probe failed to detect any rearranged band in the first DNA sample. No rearrangement was identified at the c-myc and N-ras loci, while a slight c-myc amplification was evident in both DNA samples tested.
我们在此描述一名Ph'阳性慢性粒细胞白血病（CML）患者，其发生了Ph'阴性母细胞危象。在母细胞期开始时以及结束时，即患者死亡前不久，在两个不同时间点对母细胞DNA进行了分析。尽管两个样本的细胞均无Ph'染色体标记（甚至没有隐匿型的），但使用“3'-bcr”探针，我们在第二个DNA样本中检测到了bcr基因重排。相同探针和“5'-bcr”探针在第一个DNA样本中未检测到任何重排条带。在c-myc和N-ras基因座未发现重排，而在两个检测的DNA样本中均明显存在轻微的c-myc扩增。
