Frączak Ewa, Olbromski Mateusz, Piotrowska Aleksandra, Glatzel-Plucińska Natalia, Dzięgiel Piotr, Dybko Jarosław, Kuliczkowski Kazimierz, Wróbel Tomasz
Department of Hematology, Blood Neoplasms and Bone Marrow Transplantation, Medical University, Wrocław, Poland.
Department of Histology and Embryology, Medical University, Wrocław, Poland.
Acta Histochem. 2018 Jan;120(1):22-27. doi: 10.1016/j.acthis.2017.10.010. Epub 2017 Nov 14.
Bone marrow adipocytes (BMAs) derived from mesenchymal stem cells (MSC) are an active and significant element of the bone marrow microenvironment. They are involved in metabolic functions, complex interactions with other stromal cells, and in the development and progression of tumours. Currently, there is little data regarding the role of BMAs in haematological malignancies. Due to this, we have attempted to characterise the BMAs in these malignancies in terms of quantity and morphology. Our study included 30 patients aged 22-76 with myelo- (n=17) and lymphoproliferative malignancies (n=13), both with and without bone marrow infiltration. Trepanobioptate was the evaluated material. The number and diameter of BMAs were measured, and the percentage of adipocytes (adipocyte fraction - AF), hematopoietic cells (hematopoietic fraction - HF) and trabecular bone (trabecular bone fraction - BF) was calculated. The obtained results were considered against the clinical parameters of age, sex, body weight, body surface area (BSA) and body mass index (BMI). We observed that as age increases, the number of BMA/mm, the diameter of adipocytes and AF increase while BF and HF decrease. However, this relationship was not statistically significant. A significant correlation of BMA parameters was also not found in relation to weight, BMI and BSA, and the number and diameter of BMAs were comparable in both sexes. The trepanobioptate of infiltrated bone marrow showed a decreased number of BMA/mm compared to the trepanobioptate from bone marrow without infiltration (97.44±69.16 vs. 164.14±54.16; p=0.010) with a marked difference in men (69.75±65.26 vs. 180.33±60.40; p=0.007). These trepanobioptate also showed an increase in the number of BMA/mm with age (r=0.472; p=0.041), and with an increase of BMI, an increase in diameter of BMAs (r=0.625; p=0.007) and AF (r=0.546; p=0.023). The number and size of BMAs, as well as AF, BF and HF in patients with myeloproliferative malignancies did not differ significantly compared to patients with lymphoproliferative malignancies.
源自间充质干细胞（MSC）的骨髓脂肪细胞（BMA）是骨髓微环境中一个活跃且重要的组成部分。它们参与代谢功能、与其他基质细胞的复杂相互作用以及肿瘤的发生和发展。目前，关于BMA在血液系统恶性肿瘤中的作用的数据很少。因此，我们试图从数量和形态方面对这些恶性肿瘤中的BMA进行特征描述。我们的研究纳入了30例年龄在22 - 76岁之间的患者，其中17例为骨髓增殖性疾病，13例为淋巴细胞增殖性疾病，均伴有或不伴有骨髓浸润。环钻活检组织为评估材料。测量了BMA的数量和直径，并计算了脂肪细胞百分比（脂肪细胞分数 - AF）、造血细胞百分比（造血细胞分数 - HF）和小梁骨百分比（小梁骨分数 - BF）。将获得的结果与年龄、性别、体重、体表面积（BSA）和体重指数（BMI）的临床参数进行对照分析。我们观察到，随着年龄的增加，每平方毫米BMA的数量、脂肪细胞直径和AF增加，而BF和HF减少。然而，这种关系在统计学上并不显著。在体重、BMI和BSA方面，也未发现BMA参数之间存在显著相关性，并且BMA的数量和直径在两性之间具有可比性。与无浸润骨髓的环钻活检组织相比，浸润骨髓的环钻活检组织每平方毫米BMA的数量减少（97.44±69.16对164.14±54.16；p = 0.010），男性之间存在显著差异（69.75±65.26对180.33±60.40；p = 0.007）。这些环钻活检组织还显示，每平方毫米BMA的数量随年龄增加而增加（r = 0.472；p = 0.041），并且随着BMI的增加，BMA直径（r = 0.625；p = 0.007）和AF（r = 0.546；p = 0.023）增加。与淋巴细胞增殖性疾病患者相比，骨髓增殖性疾病患者的BMA数量和大小以及AF、BF和HF没有显著差异。
