State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China.
Department of Microbiology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China.
Microb Pathog. 2018 Jan;114:176-179. doi: 10.1016/j.micpath.2017.11.020. Epub 2017 Nov 14.
Methicillin-resistant S. aureus (MRSA) has attracted more and more attention in recent years, especially in burn medical centers. Here we conducted a 5-year period study to evaluate the MRSA infection in our burn center. The staphylococcal chromosomal cassette mec (SCCmec) typing, antimicrobials susceptibility and virulence profiles were also performed among the MRSA isolates. Of the 259 S. aureus isolates, 239 (92.28%) isolates were identified as MRSA. A decreased trend of MRSA isolation rate over time was found (P = 0.0063). Majority of MRSA isolates in our center belonged to SCCmec type III (230/239, 96.23%). Antimicrobials susceptibility tests of the MRSA isolates revealed significantly decreased resistance to clindamycin (P = 0.0183), and increased resistance to chloramphenicol (P = 0.0020) and minocycline (P < 0.0001) over time. Trimethoprim/sulfamethoxazole, clindamycin, vancomycin, teicoplanin and linezolid were suggested to be good choice for MRSA infection in our center. Virulence factors profiling showed that most of MRSA isolates in our center carried the virulence factor pattern of cna-clfA-clfB-eno-fib-icaA-icaD-sea-psmα-lukED-hlg-hlgv-hla-hld (214/239, 89.54%). In conclusion, our study suggests that MRSA infection is serious in our burn center, but presented decreased trend over time. Most of MRSA isolates in our center presented the same virulence factor profile. More attention should be attached to nosocomial infection in burn medical center. Antimicrobials susceptibility changing over time was observed. Antimicrobials susceptibility monitoring is necessary and helps to select appropriate drugs against MRSA infections.
耐甲氧西林金黄色葡萄球菌（MRSA）近年来受到越来越多的关注，尤其是在烧伤医学中心。在这里，我们进行了一项为期 5 年的研究，以评估我们烧伤中心的 MRSA 感染情况。还对 MRSA 分离株进行了葡萄球菌染色体盒 mec（SCCmec）分型、抗菌药物敏感性和毒力谱分析。在 259 株金黄色葡萄球菌分离株中，239 株（92.28%）被鉴定为 MRSA。随着时间的推移，MRSA 分离率呈下降趋势（P=0.0063）。我们中心的大多数 MRSA 分离株属于 SCCmec 型 III（230/239，96.23%）。MRSA 分离株的抗菌药物敏感性试验显示，克林霉素的耐药性显著降低（P=0.0183），氯霉素（P=0.0020）和米诺环素（P<0.0001）的耐药性随着时间的推移而增加。复方磺胺甲噁唑、克林霉素、万古霉素、替考拉宁和利奈唑胺被建议作为我们中心治疗 MRSA 感染的良好选择。毒力因子分析显示，我们中心的大多数 MRSA 分离株携带 cna-clfA-clfB-eno-fib-icaA-icaD-sea-psmα-lukED-hlg-hlgv-hla-hld 的毒力因子模式（214/239，89.54%）。总之，我们的研究表明，MRSA 感染在我们的烧伤中心很严重，但随着时间的推移呈下降趋势。我们中心的大多数 MRSA 分离株呈现出相同的毒力因子谱。应更加关注烧伤医学中心的医院感染。随着时间的推移，观察到抗菌药物敏感性的变化。抗菌药物敏感性监测是必要的，可以帮助选择合适的药物治疗 MRSA 感染。
