尼日利亚西北部比尔宁凯比联邦医疗中心金黄色葡萄球菌临床分离株中酚溶性调节素-mec（PSM-mec）的分子检测
Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria.
作者信息
Maishanu Isah Musa, Gbonjubola Adeshina O, Mujahid Hussaini, Olayinka Busayo O
机构信息
Kebbi State University of Science and Technology Aliero, Nigeria.
Ahmadu Bello University Zaria, Kaduna, Nigeria.
出版信息
GMS Hyg Infect Control. 2025 Mar 6;20:Doc09. doi: 10.3205/dgkh000538. eCollection 2025.
AIM
This study was carried out to isolate and detect virulence genes associated with clinical isolates from the Federal Medical Center Birnin Kebbi, Nigeria.
METHODS
To obtain isolates, samples were taken from urine, sputum, blood and wound sources. was phenotypically identified using Microgen staph ID system and PSM-mec and PVL genes were detected using polymerase chain reaction (PCR).
RESULTS
A total of 48 non-duplicate isolates were obtained (21 from wound swabs, 7 from blood, 15 from urine, and 5 from sputum). From the 14 isolates examined by PCR, the most abundant gene was (42.8%), while the PVL was the least abundant with 21.4%.
CONCLUSION
Because it gives highly specific and accurate results, it is essential to use the PCR technique to detect virulence determinants as well as PSM-mec and PVL as targets for antimicrobial agents.
目的
本研究旨在从尼日利亚比尔宁凯比联邦医疗中心的临床分离株中分离并检测毒力基因。
方法
为获取分离株，从尿液、痰液、血液和伤口来源采集样本。使用Microgen葡萄球菌鉴定系统进行表型鉴定，并使用聚合酶链反应（PCR）检测PSM-mec和PVL基因。
结果
共获得48株非重复分离株（21株来自伤口拭子，7株来自血液，15株来自尿液，5株来自痰液）。在通过PCR检测的14株分离株中，最丰富的基因是[此处原文缺失具体基因名称]（42.8%），而PVL最少，为21.4%。
结论
由于PCR技术能给出高度特异且准确的结果，使用该技术检测毒力决定因素以及将PSM-mec和PVL作为抗菌药物的靶点至关重要。
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