Rhodes J M, Black R R, Savage A
Department of Medicine, Queen Elizabeth Hospital, Birmingham, U.K.
Dig Dis Sci. 1988 Nov;33(11):1359-63. doi: 10.1007/BF01536988.
Evidence is accumulating that colonic mucin glycoconjugates are altered in ulcerative colitis. In order to investigate this further, the lectin-binding properties of rectal glycoconjugates have been studied in ulcerative colitis, Crohn's disease, and controls using lectin-peroxidase histochemistry. Ten lectins were used including peanut agglutinin (PNA) which is known to bind to malignant and adenomatous but not normal colonic mucins. Eight of 21 ulcerative colitis rectal biopsies and 10 of 17 Crohn's disease rectal biopsies showed PNA positivity, particularly in the supranuclear region of surface epithelial cells. There was no correlation between PNA positivity and duration of disease or inflammation, and none of the biopsies showed evidence of dysplasia. This abnormality in epithelial cell glycoconjugates seems to be commonly present in nondysplastic mucosa and occurs in both ulcerative colitis and Crohn's disease. It may reflect a fundamental abnormality in mucus glycoprotein synthesis in inflammatory bowel disease.
越来越多的证据表明，溃疡性结肠炎患者结肠黏蛋白糖缀合物会发生改变。为了进一步研究这一现象，利用凝集素-过氧化物酶组织化学方法，对溃疡性结肠炎、克罗恩病患者以及对照组直肠糖缀合物的凝集素结合特性进行了研究。使用了10种凝集素，其中包括花生凝集素（PNA），已知它能与恶性和腺瘤性而非正常结肠黏蛋白结合。21例溃疡性结肠炎直肠活检标本中有8例、17例克罗恩病直肠活检标本中有10例显示PNA阳性，尤其在表面上皮细胞的核上区。PNA阳性与疾病持续时间或炎症之间无相关性，且所有活检标本均未显示发育异常的证据。上皮细胞糖缀合物的这种异常似乎常见于无发育异常的黏膜中，且在溃疡性结肠炎和克罗恩病中均会出现。它可能反映了炎症性肠病中黏液糖蛋白合成的一种基本异常。
