Zhuo Song-Ming, Li Si-Cong, Lin Yong-Qun, Yu Hai-Bin, Li Na
Department of Respiratory Medicine, the Affiliated Shenzhen Longgang Center Hospital, Zunyi Medical University, Shenzhen City, Guangdong Province, China.
Zhuhai Campus of Zunyi Medical University, Zhuhai City, Guangdong Province, China.
Iran J Basic Med Sci. 2017 Oct;20(10):1102-1108. doi: 10.22038/IJBMS.2017.9367.
In this study, we aimed to investigate the effects of anti-Fas ribozyme on the apoptosis of T lymphocytes (T cells) in mice model with chronic obstructive pulmonary disease (COPD).
Male 6-week-old C57BL/6 mice were used to establish the COPD model by exposure to cigarette smoke. The COPD mice were sacrificed for spleen dissection and T cell isolation. T cells were randomly divided into four groups (n=10 per group). Group A was used as the control. B, C, and D groups were transfected with empty lentivirus, anti-Fas ribozyme, and an anti-Fas ribozyme mutant, respectively. The expression of Fas mRNA and protein in the T cells were evaluated using qPCR and Western blot, respectively. Flow cytometry was used to evaluate the apoptosis of CD T cells and calculate the ratio of CD to CD T cells (CD/CD).
Anti-Fas ribozyme significantly inhibited the expression of Fas in the T cells of COPD mice. In addition, the number of apoptotic CD T cells and CD/CD of the C and D groups were significantly lower and higher than those of group A, respectively (<0.05). The apoptotic CD T cells and CD CD of the C group were significantly lower and higher than those of group D, respectively (<0.05).
Anti-Fas ribozyme significantly inhibited the expression of Fas, increased CD/CD, and inhibited the apoptosis of T cells in COPD mice.
在本研究中，我们旨在探讨抗Fas核酶对慢性阻塞性肺疾病（COPD）小鼠模型中T淋巴细胞（T细胞）凋亡的影响。
使用6周龄雄性C57BL/6小鼠，通过暴露于香烟烟雾建立COPD模型。处死COPD小鼠以解剖脾脏并分离T细胞。将T细胞随机分为四组（每组n = 10）。A组作为对照。B、C和D组分别用空慢病毒、抗Fas核酶和抗Fas核酶突变体转染。分别使用qPCR和蛋白质印迹法评估T细胞中Fas mRNA和蛋白质的表达。使用流式细胞术评估CD T细胞的凋亡，并计算CD与CD T细胞的比例（CD/CD）。
抗Fas核酶显著抑制COPD小鼠T细胞中Fas的表达。此外，C组和D组凋亡CD T细胞的数量和CD/CD分别显著低于和高于A组（<0.05）。C组凋亡CD T细胞和CD CD分别显著低于和高于D组（<0.05）。
抗Fas核酶显著抑制Fas的表达，增加CD/CD，并抑制COPD小鼠T细胞的凋亡。
