用于未官能化环烷烃胺化的生物催化级联反应。
A biocatalytic cascade for the amination of unfunctionalised cycloalkanes.
作者信息
Tavanti Michele, Mangas-Sanchez Juan, Montgomery Sarah L, Thompson Matthew P, Turner Nicholas J
机构信息
School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK.
出版信息
Org Biomol Chem. 2017 Nov 29;15(46):9790-9793. doi: 10.1039/c7ob02569f.
Here we describe a one-pot, three-enzyme, cascade involving a cytochrome P450 monooxygenase, an alcohol dehydrogenase and a reductive aminase for the synthesis of secondary amines from cycloalkanes. Amine product concentrations of up to 19.6 mM were achieved. The preparative scale amination of cyclohexane was also demonstrated with a space-time yield of 2 g L d.
在此，我们描述了一种一锅三酶级联反应，该反应涉及一种细胞色素P450单加氧酶、一种醇脱氢酶和一种还原胺化酶，用于从环烷烃合成仲胺。实现了高达19.6 mM的胺产物浓度。还展示了环己烷的制备规模胺化反应，时空产率为2 g L⁻¹ d⁻¹。
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