Higgins M L, Glaser D, Dicker D T, Zito E T
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
J Bacteriol. 1989 Jan;171(1):349-52. doi: 10.1128/jb.171.1.349-352.1989.
Segregation was studied by measuring the positions of autoradiographic grain clusters in chains formed from single cells containing on average less than one radiolabeled chromosome strand. The degree to which chromosomal and cell wall material cosegregated was quantified by using the methods of S. Cooper and M. Weinberger, dividing the number of chains labeled at the middle. This analysis indicated that in contrast to chromosomal segregation in Escherichia coli and, in some studies, to that in gram-positive rods, chromosomal segregation in Streptococcus faecium was slightly nonrandom and did not vary with growth rate. Results were not significantly affected by strand exchange. In contrast, labeled cell wall segregated predominantly nonrandomly.
通过测量由平均含有少于一条放射性标记染色体链的单细胞形成的链中放射自显影颗粒簇的位置来研究分离情况。使用S. 库珀和M. 温伯格的方法，通过划分在中间标记的链的数量，对染色体和细胞壁物质共分离的程度进行了量化。该分析表明，与大肠杆菌中的染色体分离以及在一些研究中革兰氏阳性杆菌中的染色体分离不同，粪肠球菌中的染色体分离略有非随机性，并且不随生长速率而变化。结果不受链交换的显著影响。相比之下，标记的细胞壁主要以非随机方式分离。
