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粪肠球菌指数生长期细胞浮力密度的细胞周期变化。

Cell cycle changes in the buoyant density of exponential-phase cells of Streptococcus faecium.

作者信息

Dicker D T, Higgins M L

出版信息

J Bacteriol. 1987 Mar;169(3):1200-4. doi: 10.1128/jb.169.3.1200-1204.1987.

Abstract

Cell buoyant densities were determined by centrifugation in Percoll gradients containing exponential-phase cells of Streptococcus faecium ATCC 9790 grown at a mass doubling time of about 33 min. This bacterium showed the highest average density values (1.13 g/ml) measured to date for any eucaryotic or procaryotic organism. Fractions having the highest densities were enriched with cells that were in the process of dividing or had just divided. These high-density fractions were also enriched with cells that had newly initiated sites of cell wall growth. It appears that S. faecium shows minimum cell densities in the midportion of its cycle.

摘要

通过在含有粪肠球菌ATCC 9790指数生长期细胞的Percoll梯度中离心来测定细胞的浮力密度,粪肠球菌ATCC 9790在约33分钟的质量倍增时间下生长。对于任何真核或原核生物,该细菌显示出迄今为止测得的最高平均密度值(1.13 g/ml)。具有最高密度的组分富含正在分裂或刚分裂的细胞。这些高密度组分还富含新启动细胞壁生长位点的细胞。粪肠球菌似乎在其细胞周期的中期显示出最低的细胞密度。

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