解淀粉芽孢杆菌胞外核糖核酸酶的翻译与加工
Translation and processing of Bacillus amyloliquefaciens extracellular RNase.
作者信息
Paddon C J, Vasantha N, Hartley R W
机构信息
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
出版信息
J Bacteriol. 1989 Feb;171(2):1185-7. doi: 10.1128/jb.171.2.1185-1187.1989.
Abstract
Bacillus amyloliquefaciens extracellular RNase has been previously cloned and expressed in Bacillus subtilis. Site-specific mutagenesis experiments have identified codon -39 as the start site of translation. We have determined the primary signal peptide cleavage site of preprobarnase and propose a pathway for the conversion of probarnase to mature barnase.
摘要
解淀粉芽孢杆菌细胞外核糖核酸酶先前已在枯草芽孢杆菌中克隆并表达。定点诱变实验已确定密码子-39为翻译起始位点。我们已确定前原核糖核酸酶的主要信号肽切割位点，并提出了原核糖核酸酶转化为成熟核糖核酸酶的途径。
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