Pond J L, Eddy C K, Mackenzie K F, Conway T, Borecky D J, Ingram L O
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
J Bacteriol. 1989 Feb;171(2):767-74. doi: 10.1128/jb.171.2.767-774.1989.
The Zymomonas mobilis gene encoding acid phosphatase, phoC, has been cloned and sequenced. The gene spans 792 base pairs and encodes an Mr 28,988 polypeptide. This protein was identified as the principal acid phosphatase activity in Z. mobilis by using zymograms and was more active with magnesium ions than with zinc ions. Its promoter region was similar to the -35 "pho box" region of the Escherichia coli pho genes as well as the regulatory sequences for Saccharomyces cerevisiae acid phosphatase (PHO5). A comparison of the gene structure of phoC with that of highly expressed Z. mobilis genes revealed that promoters for all genes were similar in degree of conservation of spacing and identity with the proposed Z. mobilis consensus sequence in the -10 region. The phoC gene contained a 5' transcribed terminus which was AT rich, a weak ribosome-binding site, and less biased codon usage than the highly expressed Z. mobilis genes.
运动发酵单胞菌编码酸性磷酸酶的基因phoC已被克隆和测序。该基因跨度为792个碱基对，编码一个分子量为28,988的多肽。通过酶谱分析，该蛋白被鉴定为运动发酵单胞菌中的主要酸性磷酸酶活性，并且其对镁离子的活性比对锌离子的活性更高。其启动子区域与大肠杆菌pho基因的-35“pho盒”区域以及酿酒酵母酸性磷酸酶（PHO5）的调控序列相似。将phoC的基因结构与高表达的运动发酵单胞菌基因的结构进行比较发现，所有基因的启动子在间隔保守程度以及与运动发酵单胞菌在-10区域的推测共有序列的一致性方面都相似。phoC基因包含一个富含AT的5'转录末端、一个较弱的核糖体结合位点，并且密码子使用偏好性比高表达的运动发酵单胞菌基因小。
