U Gandhi Neha, B Chandra Sathees
Department of Biological, Chemical and Physical Sciences, Roosevelt University, Chicago, USA.
Acta Inform Med. 2012 Sep;20(3):167-73. doi: 10.5455/aim.2012.20.167-173.
Bacterial nonspecific acid phosphohydrolases (NSAPs) or phosphatases are group of enzymes secreted as soluble periplasmic proteins or retained as membrane bound lipoproteins that are usually able to dephosphorylate a broad array of structurally unrelated organic phosphoesters (nucleotides, sugar phosphates, phytic acid etc.) to acquire inorganic phosphate (Pi) and organic byproducts. They exhibit optimal catalytic activity at acidic to neutral pH values. On the basis of amino acid sequence relatedness, phosphatase are grouped into different molecular families namely Class A, Class B and Class C acid phosphatase respectively.
In this article out of thirty three sequences, twenty six belonging to each of the three classes of bacterial acid phosphatase and seven belonging to archaeal phosphoesterases were analyzed using various tools of bioinformatics. Phylogenetic analysis, dot plot comparisons and motif analysis were done to identify a number of similarities and differences between three classes of bacterial acid phosphatases and archaeal phosphoesterases. In this research we have attempted to decipher evolutionary relationship between three classes of bacterial acid phosphatase and archaeal phosphoesterases using bioinformatics approach.
细菌非特异性酸性磷酸水解酶（NSAPs）或磷酸酶是一类作为可溶性周质蛋白分泌或作为膜结合脂蛋白保留的酶，它们通常能够使多种结构不相关的有机磷酸酯（核苷酸、糖磷酸酯、植酸等）去磷酸化，以获取无机磷酸盐（Pi）和有机副产物。它们在酸性至中性pH值下表现出最佳催化活性。基于氨基酸序列相关性，磷酸酶分别被分为不同的分子家族，即A类、B类和C类酸性磷酸酶。
在本文中，使用各种生物信息学工具对33个序列进行了分析，其中26个属于三类细菌酸性磷酸酶中的每一类，7个属于古细菌磷酸酯酶。进行了系统发育分析、点图比较和基序分析，以确定三类细菌酸性磷酸酶和古细菌磷酸酯酶之间的一些异同。在本研究中，我们试图使用生物信息学方法来解读三类细菌酸性磷酸酶和古细菌磷酸酯酶之间的进化关系。
