Comai L, Baden C S, Harada J J
Department of Botany, University of California, Davis 95616.
J Biol Chem. 1989 Feb 15;264(5):2778-82.
We analyzed five malate synthase cDNA clones from the higher plant Brassica napus L. We determined the complete mRNA sequence and showed that the longest cDNA clone, pMS1, contains the entire protein coding region. The deduced polypeptide consists of 561 amino acids with a molecular mass of 63,700 daltons. To discern whether the cloned mRNAs represent distinct malate synthase polypeptides, we compared restriction maps and partial nucleotide sequence of the cDNA clones as well as their pattern of hybridization with restriction fragments in nuclear DNA. The results suggest that the five cloned mRNAs are encoded by either a single gene or by highly conserved members of the gene family.
我们分析了来自高等植物甘蓝型油菜的五个苹果酸合酶cDNA克隆。我们确定了完整的mRNA序列，并表明最长的cDNA克隆pMS1包含整个蛋白质编码区。推导的多肽由561个氨基酸组成，分子量为63,700道尔顿。为了辨别克隆的mRNA是否代表不同的苹果酸合酶多肽，我们比较了cDNA克隆的限制性图谱和部分核苷酸序列，以及它们与核DNA中限制性片段的杂交模式。结果表明，这五个克隆的mRNA要么由单个基因编码，要么由基因家族中高度保守的成员编码。
