Département de sciences biologiques, Université de Montréal, Montréal, Québec, Canada.
Ministère des Forêts, de la Faune et des Parcs, Gouvernement du Québec, Québec, Canada.
J Hered. 2018 May 11;109(4):405-415. doi: 10.1093/jhered/esx103.
Mating system characteristics are of great importance as they may influence male and female reproductive success and reproductive isolation. The wood turtle (Glyptemys insculpta) is a terrestrial freshwater species listed as endangered by the International Union for Conservation of Nature. Considering its conservation status and the paucity of information currently available on parentage relationship for the species, we performed a microsatellite analysis to study the mating system of wood turtles in the Shawinigan River (Québec). We sampled 38 clutches over 2 years (14 in 2006 and 24 in 2007), for a total of 248 offspring genotyped with 7 microsatellite loci. The reconstructed genotypes of the fathers revealed that reproductive success in the sampled clutches varied greatly between males and are positively correlated with the number of mates and clutches sired. Frequency of multiple paternity was estimated at 37% through a consensus of 3 different estimation methods. Positive correlation was observed between the genetic diversity of clutches and the number of fathers. Repeat paternity, however, was observed in 88% of the clutches by the same female in successive years, which suggests either a frequent use of sperm storage, or remating with the same partner in successive years.
交配系统特征非常重要，因为它们可能影响雌雄繁殖成功和生殖隔离。木雕龟（Glyptemys insculpta）是一种陆生淡水物种，被国际自然保护联盟列为濒危物种。考虑到其保护状况以及目前有关该物种亲子关系的信息匮乏，我们进行了微卫星分析，以研究沙威尼根河（魁北克）的木雕龟交配系统。我们在两年内（2006 年 14 个，2007 年 24 个）采集了 38 窝卵，共对 248 只后代进行了 7 个微卫星位点的基因型分析。对父亲的重建基因型表明，样本卵窝中的繁殖成功率在雄性之间差异很大，并且与配偶数量和窝数呈正相关。通过 3 种不同估计方法的共识，估计多父本的频率为 37%。巢的遗传多样性与父亲的数量之间存在正相关关系。然而，同一位雌性在连续几年中观察到了重复父本现象，这表明雌性可能经常使用精子储存，或者在连续几年中与同一伴侣交配。
