Fitzsimmons N N
Centre for Conservation Biology, Department of Zoology, University of Queensland, Brisbane, Australia.
Mol Ecol. 1998 May;7(5):575-84. doi: 10.1046/j.1365-294x.1998.00355.x.
Paternity of 22 green turtle (Chelonia mydas) clutches from 13 females of the southern Great Barrier Reef breeding population was determined through microsatellite analyses at five loci, including the analysis of successive clutches for nine of the females. A large number of alleles per locus (10-40) provided probabilities of detecting multiple paternity that were quite high, particularly at all loci combined (99.9%). Although green turtles are promiscuous breeders and there was an expectation of finding extensive multiple paternity, only two clutches were multiply sired and, in these, very few eggs had been fertilized by a secondary male. The rarity of multiple paternity may reflect either a low proportion of multiple matings by females in this population, or sperm competition, possibly resulting from a first-male sperm preference. Additionally, the analysis of > 900 offspring provided data on mutations, which included 20 mutation events that were observed in 27 offspring and involved both maternal and paternal lineages. Most mutations (n = 16) occurred at a single highly variable locus and their presence emphasizes the need to use multiple loci in paternity studies.
通过对包括大堡礁南部繁殖种群的13只雌性绿海龟（蠵龟）所产的22窝卵进行5个微卫星位点的分析，确定了其亲权关系，其中对9只雌性海龟的连续窝卵进行了分析。每个位点有大量等位基因（10 - 40个），这使得检测多重父权的概率相当高，尤其是所有位点综合起来时（99.9%）。尽管绿海龟是滥交繁殖者，人们预期会发现广泛的多重父权现象，但只有两窝卵是由多个雄性授精的，而且在这些卵中，只有极少数是由第二只雄性授精的。多重父权现象的罕见可能反映出该种群中雌性多次交配的比例较低，或者反映出精子竞争，这可能是由于优先选择第一只雄性的精子所致。此外，对900多个后代的分析提供了有关突变的数据，其中在27个后代中观察到20个突变事件，涉及母系和父系谱系。大多数突变（n = 16）发生在一个高度可变的位点，它们的存在强调了在亲权研究中使用多个位点的必要性。
