Badr Yassien, Okada Ayaka, Abo-Sakaya Rania, Beshir Emad, Ohya Kenji, Fukushi Hideto
Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
Department of Animal Medicine, Faculty of Veterinary Medicine, Damanhour University, El-Beheira, Egypt.
Arch Virol. 2018 Mar;163(3):599-607. doi: 10.1007/s00705-017-3650-4. Epub 2017 Nov 17.
Equine herpesvirus type 1 (EHV-1) UL11 is a 74-amino-acid tegument protein encoded by ORF51 of the EHV-1 genome. EHV-1 UL11 was previously reported by other researchers using the RacL22 and RacH strains to be nonessential for viral replication in cultured cells. Here, we constructed UL11 mutant viruses including a UL11 null mutant and three C-terminal truncated mutants, for further characterization of EHV-1 UL11 using bacterial artificial chromosome (BAC) technology based on the neuropathogenic strain Ab4p. EHV-1 Ab4p UL11 was localized to juxtanuclear and Golgi regions as reported by other researchers. We found that no progeny viruses were produced by transfection of fetal equine kidney cells and rabbit kidney (RK-13) cells with the UL11 null mutant and truncation mutant BAC DNAs. However, mutant viruses were generated after transfection of RK13-UL11 cells constitutively expressing EHV-1 UL11 with the mutant BAC DNAs. In conclusion, UL11 of EHV-1 Ab4p is essential for replication in cultured cells.
马疱疹病毒1型（EHV-1）UL11是一种由EHV-1基因组的开放阅读框51编码的74个氨基酸的被膜蛋白。其他研究人员先前使用RacL22和RacH毒株报道，EHV-1 UL11对培养细胞中的病毒复制并非必需。在此，我们构建了包括UL11缺失突变体和三个C端截短突变体的UL11突变病毒，以基于神经致病株Ab4p利用细菌人工染色体（BAC）技术进一步鉴定EHV-1 UL11。如其他研究人员所报道，EHV-1 Ab4p UL11定位于核周和高尔基体区域。我们发现，用UL11缺失突变体和截短突变体BAC DNA转染马胎儿肾细胞和兔肾（RK-13）细胞后未产生子代病毒。然而，用突变BAC DNA转染组成性表达EHV-1 UL11的RK13-UL11细胞后产生了突变病毒。总之，EHV-1 Ab4p的UL11对培养细胞中的复制至关重要。
