Lee Hei Hwa, Chen Suen Chi, Lee Jong Feng, Lin Hsin Yu, Chen Bai Hsiun
Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Forensic Sci Int. 2018 Jan;282:35-40. doi: 10.1016/j.forsciint.2017.11.006. Epub 2017 Nov 11.
According to domestic and international epidemiological investigation, the proportion of substance involved sexual assault has the trend of ascent. In the past, laboratory methods that investigated urine sample of the sexual assault victims was to screen with enzyme immunoassay and then confirmed with mass spectrometry. The objective of the study is to simultaneously identify abused drugs in 126 decoded urine samples of sexual assault victims by liquid chromatography tandem mass spectrometry. The instrument was operated in multiple-reaction monitoring with an electro-spray positive ionization mode. Chromatograms were separated with ACE5 C18 column on a gradient of acetonitrile. After liquid-liquid extraction, samples were passed through a 0.22μm PVDF filter before injection into the system. The limits of quantitation ranged from 0.2 to 10ng/mL. The precision (CV) results were below 12.9% (intraday) and 15.0% (interday). The intraday accuracy ranged from 84.8 to 121.0%, interday accuracy ranged from 72.0 to 117.3%. We found that 29 (23.0%) were positive for drugs. The most common drug identified is flunitrazepam (11.1%), followed by nimetazepam and ketamine (7.9%), some new psychoactive substances, such as 2C-B, mephedrone, methylone, PMA and PMMA were also identified. We identified abused drugs, benzodiazepines, and new psychoactive substances in urine of sexual assault victims by using liquid chromatography tandem mass spectrometry.
根据国内外流行病学调查，涉及毒品的性侵犯比例呈上升趋势。过去，对性侵犯受害者尿液样本进行检测的实验室方法是先用酶免疫分析法进行筛查，然后用质谱法进行确证。本研究的目的是通过液相色谱串联质谱法同时鉴定126份性侵犯受害者尿液样本中的滥用药物。仪器采用电喷雾正离子模式在多反应监测下运行。色谱图在ACE5 C18柱上以乙腈梯度分离。液液萃取后，样品通过0.22μm PVDF滤膜，然后注入系统。定量限为0.2至10ng/mL。精密度（CV）结果日内低于12.9%，日间低于15.0%。日内准确度范围为84.8%至121.0%，日间准确度范围为72.0%至117.3%。我们发现29份（23.0%）样本药物检测呈阳性。鉴定出的最常见药物是氟硝西泮（11.1%），其次是硝甲西泮和氯胺酮（7.9%），还鉴定出了一些新型精神活性物质，如2C-B、甲麻黄碱、亚甲基二氧甲基苯丙胺、3,4-亚甲基二氧基苯丙胺和聚甲基丙烯酸甲酯。我们通过液相色谱串联质谱法鉴定了性侵犯受害者尿液中的滥用药物、苯二氮卓类药物和新型精神活性物质。
