A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Street 8, Kazan, 420088, Russian Federation.
A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Street 8, Kazan, 420088, Russian Federation.
Colloids Surf B Biointerfaces. 2018 Feb 1;162:52-59. doi: 10.1016/j.colsurfb.2017.10.070. Epub 2017 Nov 11.
The present work introduces an impact of polyelectrolyte-based hydrophilic shell on magnetic relaxivity and luminescence of hard cores built from isostructural complexes of Tb(III) and Gd(III) in the core-shell aqueous colloids. Microscopic and scattering techniques reveal "plum pudding" morphology of the colloids, where polyelectrolyte-coated ultrasmall (<5nm) hard cores form aggregates in aqueous solutions. Interaction of bovine serum albumin (BSA) with the colloids provides a tool to modify the polyelectrolyte-based shell, which is the reason for the improvement in both aggregation behavior of the colloids and their relaxivity. The modification of the hydrophilic polyelectrolyte-based shell enables to tune the longitudinal relaxivity from 5.9 to 23.3mMs at 0.47T. This tendency is the reason for significant improvement of contrasting effect of the colloids in T- and T-weighted images obtained by whole body scanner at 1.5T. High contrasting effect of the colloids, together with low cytotoxicity towards Wi-38 diploid human cells makes them promising MRI contrast agents.
本工作介绍了基于聚电解质的亲水性壳层对由结构相同的 Tb(III) 和 Gd(III) 配合物构建的核壳型水基胶体中磁性弛豫率和发光的影响。微观和散射技术揭示了胶体的“布丁”形态，其中聚电解质包覆的超小(<5nm)硬核在水溶液中形成聚集体。牛血清白蛋白(BSA)与胶体的相互作用为修饰基于聚电解质的壳层提供了一种手段，这也是胶体聚集行为及其弛豫率提高的原因。亲水聚电解质壳层的修饰能够将纵向弛豫率从 5.9 调节至 0.47T 时的 23.3mMs。这种趋势是胶体在 1.5T 全身扫描仪获得的 T 和 T 加权图像中对比效果显著改善的原因。胶体的高对比效果，加上对 Wi-38 二倍体人细胞的低细胞毒性，使它们成为有前途的 MRI 造影剂。
