Zheng Chen-Xi, Wang Sheng-Ming, Bai Yun-Hu, Luo Ting-Ting, Wang Jia-Qi, Dai Chun-Qiu, Guo Bao-Lin, Luo Shi-Cheng, Wang Dong-Hui, Yang Yan-Ling, Wang Ya-Yun
Department of Anatomy, Histology and Embryology, K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, China.
Department of Hepatobiliary Surgery, Xi-Jing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
Anat Rec (Hoboken). 2018 May;301(5):825-836. doi: 10.1002/ar.23723. Epub 2018 Jan 24.
Pain, especially chronic pain, has always been a heated point in both basic and clinical researches since it puts heavy burdens on both individuals and the whole society. A better understanding of the role of biological molecules and various ionic channels involved in pain can shed light on the mechanism under pain and advocate the development of pain management. Using viral vectors to transfer specific genes at targeted sites is a promising method for both research and clinical applications. Lentiviral vectors and adeno-associated virus (AAV) vectors which allow stable and long-term expression of transgene in non-dividing cells are widely applied in pain research. In this review, we thoroughly outline the structure, category, advantages and disadvantages and the delivery methods of lentiviral and AAV vectors. The methods through which lentiviral and AAV vectors are delivered to targeted sites are closely related with the sites, level and period of transgene expression. Focus is placed on the various delivery methods applied to deliver vectors to spinal cord and dorsal root ganglion both of which play important roles in primary nociception. Our goal is to provide insight into the features of these two viral vectors and which administration approach can be chosen for different pain researches. Anat Rec, 301:825-836, 2018. © 2017 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.
疼痛，尤其是慢性疼痛，一直是基础研究和临床研究的热点，因为它给个人和整个社会都带来了沉重负担。更好地理解参与疼痛的生物分子和各种离子通道的作用，有助于揭示疼痛的机制，并推动疼痛管理的发展。利用病毒载体在靶向位点转移特定基因是一种在研究和临床应用中都很有前景的方法。慢病毒载体和腺相关病毒（AAV）载体能够在非分裂细胞中稳定且长期地表达转基因，被广泛应用于疼痛研究。在这篇综述中，我们全面概述了慢病毒载体和AAV载体的结构、类别、优缺点以及递送方法。慢病毒载体和AAV载体递送至靶向位点的方法与转基因表达的位点、水平和时期密切相关。重点介绍了用于将载体递送至脊髓和背根神经节的各种递送方法，这两者在初级痛觉感受中都起着重要作用。我们的目标是深入了解这两种病毒载体的特点，以及针对不同的疼痛研究可以选择哪种给药途径。《解剖学记录》，301:825 - 836，2018年。© 2017作者。《解剖学记录》由威利期刊公司代表美国解剖学家协会出版。
