Yang Biwei, Feng Yu, McNally Alan, Zong Zhiyong
Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China; Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China.
Institute of Microbiology and Infection, College of Medical and Dental Science, University of Birmingham, Birmingham, UK.
Diagn Microbiol Infect Dis. 2018 Feb;90(2):139-142. doi: 10.1016/j.diagmicrobio.2017.10.007. Epub 2017 Oct 19.
Three carbapenem-resistant clinical isolates of the Enterobacter cloacae complex (ECC) were recovered from different patients in a hospital. All 3 isolates carried 2 carbapenemase genes bla and bla. A study was performed to characterize their relatedness and to investigate possible links among the patients. Whole genome sequencing revealed that the isolates were Enterobacter hormaechei and belonged to ST177 of the ECC. There were 19-142 single nucleotide polymorphisms (SNPs) between the isolates, suggesting that the isolates were likely from a central reservoir, which might have existed for some time. bla and bla were carried on 2 different IncF-type plasmids in the isolates. The 3 bla-carrying plasmids were almost identical and were self-transmissible, while the bla-carrying plasmids were only transmissible in the presence of the bla-carrying plasmid. The source of and direct links among them were not identified, suggesting a hospital transmission of a common multidrug resistant strain.
从一家医院的不同患者中分离出三株耐碳青霉烯类的阴沟肠杆菌复合体（ECC）临床菌株。所有三株菌株都携带两种碳青霉烯酶基因bla和bla。开展了一项研究以表征它们的亲缘关系，并调查患者之间可能存在的联系。全基因组测序显示，这些菌株为霍氏肠杆菌，属于ECC的ST177。菌株之间存在19 - 142个单核苷酸多态性（SNP），这表明这些菌株可能来自一个可能已存在一段时间的中央储存库。bla和bla存在于菌株中的两种不同IncF型质粒上。携带bla的3个质粒几乎相同且可自我传递，而携带bla的质粒仅在存在携带bla的质粒时才可传递。未确定它们的来源及直接联系，提示存在一种常见多重耐药菌株的医院内传播。
