Zhang Boke, Cao Manlin, He Yiqing, Liu Yiwen, Zhang Guoliang, Yang Cuixia, Du Yan, Xu Jing, Hu Jiajie, Gao Feng
Department of Molecular Biology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, PR China.
Department of Rehabilitation Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, PR China.
J Cancer. 2017 Sep 29;8(17):3522-3530. doi: 10.7150/jca.20227. eCollection 2017.
Breast cancer (BC)-derived hyaluronan (HA) can induce the formation of M2-like tumor-associated macrophages (TAMs) in tumor context. However, little is known about the correlation between circulating M2-like monocytes and plasma HA in BC patients. This study focused on evaluating the relationship between circulating M2-like monocytes and plasma HA, and further appraised the diagnostic value of them in BC. The expression of M2-like TAMs and HA was determined in pathological tissues by immunohistochemistry. Flow cytometry was used to detect the levels of circulating CD14CD204 M2-like monocytes in 81 BC patients, 45 patients with breast benign diseases, and 46 healthy subjects. The levels of HA, CEA, and CA15-3 were measured in plasma samples using chemiluminescence method. M2-like TAMs and HA expressions were elevated in BC tissues compared with benign tissues. In correspondence, the frequency of circulating CD14CD204 M2-like monocytes and the plasma HA levels were significantly higher in patients with BC than those in control groups. Importantly, there was a positive correlation between circulating M2-like monocytes and the plasma HA (Spearman r = 0.404, < 0.001). Area under receiver operating characteristic curve (ROC) for the combination of circulating M2-like monocytes and HA was 0.899 (95% CI: 0.853-0.946), which was higher than the panel of CEA and CA15-3. The frequency of circulating CD14CD204 M2-like monocytes was positively correlated to plasma HA levels. The combination of circulating CD14CD204 M2-like monocytes and plasma HA could provide considerable diagnostic value in BC.
乳腺癌（BC）衍生的透明质酸（HA）可在肿瘤环境中诱导形成M2样肿瘤相关巨噬细胞（TAM）。然而，BC患者循环中的M2样单核细胞与血浆HA之间的相关性鲜为人知。本研究聚焦于评估循环M2样单核细胞与血浆HA之间的关系，并进一步评估它们在BC中的诊断价值。通过免疫组织化学法测定病理组织中M2样TAM和HA的表达。采用流式细胞术检测81例BC患者、45例乳腺良性疾病患者和46例健康受试者循环中CD14CD204 M2样单核细胞的水平。采用化学发光法测定血浆样本中HA、癌胚抗原（CEA）和糖类抗原15-3（CA15-3）的水平。与良性组织相比，BC组织中M2样TAM和HA的表达升高。相应地，BC患者循环中CD14CD204 M2样单核细胞的频率和血浆HA水平显著高于对照组。重要的是，循环M2样单核细胞与血浆HA之间存在正相关（Spearman相关系数r = 0.404，P < 0.001）。循环M2样单核细胞与HA联合检测的受试者工作特征曲线（ROC）下面积为0.899（95%可信区间：0.853 - 0.946），高于CEA和CA15-3联合检测。循环CD14CD204 M2样单核细胞的频率与血浆HA水平呈正相关。循环CD14CD204 M2样单核细胞与血浆HA联合检测可为BC提供可观的诊断价值。
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