Krestova Michala, Hromadkova Lenka, Bilkova Zuzana, Bartos Ales, Ricny Jan
National Institute of Mental Health, Klecany, Czech Republic; Third Faculty of Medicine, Charles University, Prague, Czech Republic.
National Institute of Mental Health, Klecany, Czech Republic; Faculty of Science, Charles University, Prague, Czech Republic; Department of Biological and Biochemical Sciences, University of Pardubice, Pardubice, Czech Republic.
J Neuroimmunol. 2017 Dec 15;313:16-24. doi: 10.1016/j.jneuroim.2017.09.011. Epub 2017 Oct 6.
The presence of natural tau-reactive antibodies was reported in human blood. In this study, we isolated and characterized natural tau-reactive antibodies occurring in IVIG product Flebogamma, plasma of patients with Alzheimer's disease (AD) and older cognitively normal persons (controls). Using blotting immunoassays and ELISA, we showed reactivity of antibodies obtained from IVIG and controls against a recombinant fragment of tau (155-421aa) and aggregates present in brains of AD patients. In contrast, antibodies isolated from plasma of AD patients reacted mainly with the recombinant full-length tau form and tau monomeric forms in brain tissue.
据报道，人体血液中存在天然的tau反应性抗体。在本研究中，我们分离并鉴定了静脉注射免疫球蛋白产品Flebogamma、阿尔茨海默病（AD）患者血浆和认知正常的老年人（对照组）血浆中存在的天然tau反应性抗体。通过印迹免疫分析和酶联免疫吸附测定，我们发现从静脉注射免疫球蛋白和对照组获得的抗体对tau重组片段（155 - 421aa）以及AD患者大脑中存在的聚集体具有反应性。相比之下，从AD患者血浆中分离出的抗体主要与脑组织中的重组全长tau形式和tau单体形式发生反应。
