Ringo Aaron Edmond, Adjou Moumouni Paul Franck, Taioe Moeti, Jirapattharasate Charoonluk, Liu Mingming, Wang Guanbo, Gao Yang, Guo Huanping, Lee Seung-Hun, Zheng Weiqing, Efstratiou Artemis, Li Jixu, Inoue Noboru, Suzuki Hiroshi, Thekisoe Oriel, Xuan Xuenan
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Unit for Environmental Sciences and Management, North-West University, Potchefstroom Campus, Private Bag X6001, Potchefstroom 2520, South Africa.
Parasitol Int. 2018 Apr;67(2):144-149. doi: 10.1016/j.parint.2017.11.002. Epub 2017 Nov 15.
Tick-borne protozoan and rickettsial diseases are a major threat to livestock in tropical and sub-tropical regions of Africa. In this study we investigated the presence and distribution of Theileria spp., Babesia ovis, Anaplasma ovis, Anaplasma phagocytophilum, Ehrlichia ruminantium and SFG Rickettsia in sheep and goats from Free State and KwaZulu-Natal provinces. A total of 91 blood samples were screened in this study, 61 from goats and 30 from sheep. PCR assay was conducted using primers based on Theileria spp. 18S rRNA, Babesia ovis (BoSSU rRNA), Anaplasma ovis (AoMSP4), Anaplasma phagocytophilum epank1, Ehrlichia ruminantium pCS20 and SFG Rickettsia OmpA. Overall infection rates of Theileria spp., Anaplasma ovis and Ehrlichia ruminantium were 18 (19.8%), 33 (36.3%) and 13 (14.3%), respectively. The co-infection of two pathogens were detected in 17/91 (18.7%) of all samples, goats having higher rates of co-infection compared to sheep. Phylogenetic tree analysis sequence of pCS20 gene of E. ruminantium of this study was found to be in the same clade with Kumm2 and Riverside strains both from South Africa. The phylogram of SSU rRNA of Theileria ovis had longer branch length compared to all other sequences most of which were from Asia and Middle East. This study provides important data for understanding the tick-borne diseases occurrence in the study area and it is expected to improve the approach for the diagnosis and control of these diseases.
蜱传原生动物病和立克次体病对非洲热带和亚热带地区的家畜构成重大威胁。在本研究中，我们调查了自由邦省和夸祖鲁-纳塔尔省绵羊和山羊体内泰勒虫属、绵羊巴贝斯虫、绵羊无形体、嗜吞噬细胞无形体、反刍兽埃立克体和斑点热群立克次体的存在情况和分布。本研究共筛查了91份血样，其中61份来自山羊，30份来自绵羊。使用基于泰勒虫属18S rRNA、绵羊巴贝斯虫（BoSSU rRNA）、绵羊无形体（AoMSP4）、嗜吞噬细胞无形体epank1、反刍兽埃立克体pCS20和斑点热群立克次体OmpA的引物进行PCR检测。泰勒虫属、绵羊无形体和反刍兽埃立克体的总体感染率分别为18（19.8%）、33（36.3%）和13（14.3%）。在所有样本中有17/91（18.7%）检测到两种病原体的共同感染，山羊的共同感染率高于绵羊。本研究中反刍兽埃立克体pCS20基因的系统发育树分析序列与来自南非的Kumm2和Riverside菌株处于同一进化枝。与大多数来自亚洲和中东的所有其他序列相比，绵羊泰勒虫SSU rRNA的系统发育树分支长度更长。本研究为了解研究区域蜱传疾病的发生提供了重要数据，有望改进这些疾病的诊断和控制方法。
