Reyes-Escogido María de Lourdes, Meneses-Rodríguez David, Guardado-Mendoza Rodolfo
Metabolic Research Laboratory, Division of Health Sciences, University of Guanajuato, Campus Leon. Boulvard, Puente del Milenio No. 1001. Fracción Predio de San Carlos, León, Guanajuato 37670, México.
CONACYT - CINVESTAV-Mérida, Applied Physic Department, Carretera Antigua a Progreso, Km 6, Cordemex, Mérida, Yucatán 97310, México.
IET Nanobiotechnol. 2017 Dec;11(8):1035-1039. doi: 10.1049/iet-nbt.2017.0107.
Strains of have been used for the synthesis of metallic nanoparticles. Since the carbohydrate source could influence the yield and size of the synthesised nanoparticles, the authors evaluated the potential of 1449 and 1313 to produce silver nanoparticles (AgNPs) using three carbohydrate sources and AgNO. The presence of AgNO in the medium extended the duration of the acceleration and logarithmic phases of the two strains independently of the carbohydrate source used but did not inhibit their growth. The synthesis of AgNPs started at the second day of culture. In general, the size of the AgNPsranged from 10 to 150 nm; they were smaller and more homogeneous in lactose. In the medium supplemented with glucose, there was a lower production of nanoparticles for both strains. The AgNPs synthesised by 1313 remained enclosed in an extracellular polymeric substance, which probably played an important role in the synthesis of the nanoparticles. The carbohydrate source influenced the yield and size of the AgNPssynthesised by 1449 and 1313; the pH was also important for obtaining nanoparticles of uniform size.
菌株已被用于金属纳米颗粒的合成。由于碳水化合物来源会影响合成纳米颗粒的产量和尺寸,作者评估了1449和1313利用三种碳水化合物来源和硝酸银生产银纳米颗粒(AgNP)的潜力。培养基中硝酸银的存在延长了两种菌株加速期和对数期的持续时间,这与所使用的碳水化合物来源无关,但并未抑制它们的生长。AgNP的合成在培养的第二天开始。一般来说,AgNP的尺寸范围为10至150纳米;在乳糖中它们更小且更均匀。在补充有葡萄糖的培养基中,两种菌株的纳米颗粒产量都较低。1313合成的AgNP仍包裹在细胞外聚合物中,这可能在纳米颗粒的合成中起重要作用。碳水化合物来源影响了1449和1313合成的AgNP的产量和尺寸;pH值对于获得尺寸均匀的纳米颗粒也很重要。