Institute of Physical and Theoretical Chemistry, Institute of Biophysical Chemistry, and Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Max-von-Laue-Str. 7-9, 60438 Frankfurt, Germany.
Institute of Physical and Theoretical Chemistry, Institute of Biophysical Chemistry, and Center for Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Max-von-Laue-Str. 7-9, 60438 Frankfurt, Germany.
Prog Nucl Magn Reson Spectrosc. 2017 Nov;102-103:120-195. doi: 10.1016/j.pnmrs.2017.06.002. Epub 2017 Jul 23.
The field of dynamic nuclear polarization has undergone tremendous developments and diversification since its inception more than 6 decades ago. In this review we provide an in-depth overview of the relevant topics involved in DNP-enhanced MAS NMR spectroscopy. This includes the theoretical description of DNP mechanisms as well as of the polarization transfer pathways that can lead to a uniform or selective spreading of polarization between nuclear spins. Furthermore, we cover historical and state-of-the art aspects of dedicated instrumentation, polarizing agents, and optimization techniques for efficient MAS DNP. Finally, we present an extensive overview on applications in the fields of structural biology and materials science, which underlines that MAS DNP has moved far beyond the proof-of-concept stage and has become an important tool for research in these fields.
自 60 多年前问世以来，动态核极化领域经历了巨大的发展和多样化。在这篇综述中，我们深入概述了 DNP 增强 MAS NMR 光谱学中涉及的相关主题。这包括 DNP 机制的理论描述以及可以导致核自旋之间均匀或选择性极化扩展的极化转移途径。此外，我们还涵盖了专用仪器、极化剂和用于高效 MAS DNP 的优化技术的历史和最新发展方面。最后，我们对结构生物学和材料科学领域的应用进行了广泛的概述，这表明 MAS DNP 已经远远超出了概念验证阶段，并且已经成为这些领域研究的重要工具。
